Abstract

Reconstitutions of Transporters, Receptors, and Pathological States presents 12 lectures on the resolution and reconstitution of transporters, receptors, and pathological states. Lecture 1 discusses the reconstitution of soluble pathways, and the resolution and reconstitution of membrane complexes. Lecture 2 covers the solubilization and purification of membrane proteins. Lecture 3 explains the functions of protein and phospholipid components; the role of asymmetry; and measurement of scrambling during reconstitution. Lecture 4 presents analyses of reconstituted vesicles while Lectures 5 and 6 examine the properties of F1 and E1E2 pumps, respectively. Lecture 7 focuses on ATP-driven H+ fluxes in organelles and ATP-driven ion pumps of microorganisms and plants. Lecture 8 covers the reconstitution of the mitochondrial electron transport chain; reconstitution of photosynthetic electron transport pathways; and bacteriorhodopsin and halorhodopsin. Lecture 9 discusses the transporters of plasma membranes, mithchondria, and organelles. Lecture 10 deals with plasma membrane receptors. Lecture 11 focuses on the malignant transformation of cells while Lecture 12 speculates on the future of reconstitutions.