Abstract

Advances in Biophysical Chemistry, Volume 5, provides reviews of important topics in physical and structural biochemistry. The volume begins with a review of the chemical reactivity of DNA and its relationship to the dynamic nature of DNA conformation and its dependence on base sequence. The underlying chemistry has become extremely important to many researchers who use a host of chemical "footprinting" techniques to study biologically relevant complexes of DNA. This is followed by separate chapters that cover an innovative application of fluorescence energy transfer to investigate the dynamics of complex glycopeptides; the NMR of cations which bind to DNA, providing a picture of DNA conformation and dynamics which is complementary to that provided by 1H NMR spectroscopy; the use of NMR to study electron transfer reactions between cytochrome c peroxidase and cytochrome c; methods for analysis of data on O2 binding by hemoglobin; and experimental methods for obtaining data on protein association.