BOOK
Hematology, An Issue of Veterinary Clinics: Small Animal Practice - E-Book
(2012)
Additional Information
Book Details
Abstract
Current, important information on hematology for all small animal practitioners! Topics will include in-clinic automated hematology analyzers, quality control recommendations for point-of-care hematology analyzers, bone marrow aspiration and biopsy: indications, technique and evaluation, coombs testing and its diagnostic significance, principles and application of flow cytometry and cell sorting, hemolytic anemia due to erythrocyte enzymes deficiencies, role of hepcidin in iron metabolism and potential therapeutic applications, molecular diagnostic testing to identify hematologic malignancies, BCR-ABL in CML, a signaling pathway of initiation and transformation with potentials for targeted therapy, understanding the cause and consequences of neutropenia, hematologic abnormalities in the companion animal cancer patient, neutrophil function testing and application, application of thromboelastography to detect and monitor coagulopathies, evaluation and clinical application of platelet function testing, pathogenesis and most useful test for diagnosing and monitoring disseminated intravascular coagulation, and more!
Table of Contents
| Section Title | Page | Action | Price |
|---|---|---|---|
| Front Cover | Cover | ||
| Hematology | i | ||
| Copyright Page | ii | ||
| Table of Contents | v | ||
| Contributors | iii | ||
| Preface: Hematology | xi | ||
| Chapter 1. Automated In-Clinic Hematology Instruments for Small Animal Practitioners: What is Available, What Can They Really Do, and How Do IMake a Choice | 1 | ||
| WHAT IS AVAILABLE? | 1 | ||
| WHAT CAN IN-CLINIC HEMATOLOGY INSTRUMENTS REALLY DO? | 4 | ||
| SUMMARY | 8 | ||
| REFERENCES | 9 | ||
| Chapter 2. Quality Management Recommendations for Automated and Manual In-House Hematology of Domestic Animals | 11 | ||
| PERSONNEL TRAINING, HEALTH, AND SAFETY | 13 | ||
| MINIMIZING PREANALYTICAL ERROR | 13 | ||
| MINIMIZING POSTANALYTICAL ERROR | 21 | ||
| SUMMARY | 21 | ||
| ACKNOWLEDGMENTS | 22 | ||
| REFERENCES | 22 | ||
| Chapter 3. Bone Marrow Cytologic and Histologic Biopsies:Indications, Technique,and Evaluation | 23 | ||
| SPECIFIC INDICATIONS FOR BONE MARROW EVALUATION | 23 | ||
| CYTOLOGIC BIOPSY VERSUS HISTOLOGIC BIOPSY | 25 | ||
| SITES OF BIOPSY | 25 | ||
| BIOPSY PROCEDURES | 29 | ||
| TECHNICAL ASPECTS OF BONE MARROW PROCESSING | 30 | ||
| COMPLETE MARROW EVALUATION | 31 | ||
| Chapter 4. Coombs’ Testing and Its Diagnostic Significance in Dogs and Cats | 43 | ||
| TEST METHODOLOGY | 43 | ||
| TEST PERFORMANCE | 45 | ||
| DIAGNOSTIC SIGNIFICANCE OF THE COOMBS’ TEST IN DOGS | 46 | ||
| DIAGNOSTIC SIGNIFICANCE OF THE COOMBS’ TEST IN CATS | 47 | ||
| ALTERNATE TECHNOLOGIES FOR ANTIGLOBULIN TESTS | 49 | ||
| SUMMARY | 50 | ||
| REFERENCES | 50 | ||
| Chapter 5. Principles and Applications of Flow Cytometry and Cell Sorting in Companion Animal Medicine | 53 | ||
| BASIC PRINCIPLES AND ESSENTIAL COMPONENTS OF FLOW CYTOMETER INSTUMENTATION | 53 | ||
| FLUIDICS | 54 | ||
| EXCITATION AND COLLECTION OPTICS | 54 | ||
| DETECTING PROPERTIES OF CELLS WITH LIGHT SCATTER AND FLUORESCENCE | 56 | ||
| PRINCIPLES OF CELL SORTING | 58 | ||
| APPLICATIONS IN SMALL ANIMAL MEDICINE | 60 | ||
| FUTURE APPLICATIONS | 67 | ||
| SUMMARY | 67 | ||
| ACKNOWLEDGMENTS | 67 | ||
| REFERENCES | 67 | ||
| Chapter 6. Hemolytic Anemia in Dogs and Cats Due to Erythrocyte Enzyme Deficiencies | 73 | ||
| DIFFERENTIAL DIAGNOSIS | 73 | ||
| PYRUVATE KINASE DEFICIENCY IN DOGS AND CATS | 75 | ||
| PHOSPHOFRUCTOKINASE DEFICIENCY IN DOGS | 77 | ||
| DIAGNOSTIC TESTS | 80 | ||
| SUMMARY | 80 | ||
| ACKNOWLEDGMENTS | 80 | ||
| REFERENCES | 81 | ||
| Chapter 7. Role of Hepcidin in Iron Metabolism and Potential Clinical Applications | 85 | ||
| HEPCIDIN: NORMAL BIOLOGY | 86 | ||
| ROLE OF HEPCIDIN IN VARIOUS DISORDERS | 88 | ||
| HEPCIDIN STUDIES IN ANIMALS | 90 | ||
| LABORATORY EVALUATION OF HEPCIDIN | 90 | ||
| HEPCIDIN: CLINICAL APPLICATIONS | 91 | ||
| SUMMARY | 92 | ||
| REFERENCES | 93 | ||
| Chapter 8. Molecular Diagnostics of Hematologic Malignanciesin Small Animals | 97 | ||
| DETECTION OF MUTATIONS IN INDIVIDUAL ONCOGENES | 98 | ||
| CLONALITY ASSAYS | 101 | ||
| REFERENCES | 107 | ||
| Chapter 9. Neutropenia in Dogs and Cats: Causes and Consequences | 111 | ||
| INCREASED USE | 112 | ||
| DECREASED PRODUCTION | 112 | ||
| DESTRUCTION | 117 | ||
| NEUTROPHIL MORPHOLOGY | 117 | ||
| CONSEQUENCES | 118 | ||
| SUMMARY | 119 | ||
| REFERENCES | 119 | ||
| Chapter 10. Hematologic Abnormalitiesin the Small Animal CancerPatient | 123 | ||
| ABNORMAL DECREASES IN CIRCULATING BLOOD CELL CONCENTRATIONS | 124 | ||
| ABNORMAL INCREASES IN CIRCULATING BLOOD CELL CONCENTRATIONS | 132 | ||
| COAGULOPATHIES IN CANCER PATIENTS | 137 | ||
| DYSPROTEINEMIAS | 143 | ||
| SUMMARY | 144 | ||
| ACKNOWLEDGMENTS | 144 | ||
| REFERENCES | 144 | ||
| Chapter 11. Neutrophil Function in Small Animals | 157 | ||
| NEUTROPHIL GRANULES AND THEIR CONTENTS | 157 | ||
| NEUTROPHIL RECRUITMENT | 158 | ||
| MICROBIOCIDAL MECHANISMS | 161 | ||
| ROLE IN IMMUNE RESPONSE, INFLAMMATION, AND COAGULATION | 163 | ||
| INHERITED DISORDERS OF NEUTROPHIL FUNCTION IN VETERINARY MEDICINE | 164 | ||
| SUMMARY | 167 | ||
| REFERENCES | 167 | ||
| Chapter 12. Evaluation and Clinical Application of Platelet Function Testing in Small Animal Practice | 173 | ||
| WHOLE BLOOD ASSAYS OF PLATELET FUNCTION | 174 | ||
| ADHESION ASSAYS | 178 | ||
| PLATELET AGGREGATION ASSAYS | 179 | ||
| FLOW CYTOMETRY | 180 | ||
| FLOW CYTOMETRY: ACTIVATION-SPECIFIC CHANGES | 181 | ||
| GRANULE DISORDERS/FUNCTIONAL AND QUANTITATIVE ASSAYS | 184 | ||
| REFERENCES | 184 | ||
| Chapter 13. Laboratory Diagnosis of Disseminated Intravascular Coagulation in Dogs and Cats: The Past, the Present,and the Future | 189 | ||
| PATHOPHYSIOLOGY OF DIC | 189 | ||
| THE DIC CONTINUUM | 192 | ||
| LABORATORY TESTING OF DIC IN DOGS AND CATS | 194 | ||
| REFERENCES | 199 | ||
| Index | 203 |