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Microbiological Sensors for the Drinking Water Industry

Microbiological Sensors for the Drinking Water Industry

Bo Højris | Torben Lund Skovhus

(2018)

Additional Information

Book Details

Abstract

This most up-to date book addresses the interdisciplinary area of drinking water quality monitoring by microbiological sensors. It is edited and written by leading water professionals and experts, and binds together interests and competences within sensing technology, system behavior, business needs, legislation, education, data handling, and intelligent response algorithms. The book contains chapters on: - the history of water monitoring and early sensors - the current landscape of microbiological sensors and different measuring concepts - needs from the water industry - detailed description of several state-of-the-art sensor technologies - water industry case stories with operator experience - examples of on-line data collection and data handling - microbiological sensors applied in education and industry workshops - regulator aspects of introducing microbiological sensors It is the hope that the book will be widely used by water utility managers, technical staff working with drinking water safety, educators in the field of water quality and regulators in this field worldwide. Furthermore, that it will help bridge the gaps between these diverse and otherwise differently oriented water professionals.

Table of Contents

Section Title Page Action Price
Cover Cover
Contents v
Editorial preface xv
Foreword by Eberhard Morgenroth xix
HOW TO MAKE GOOD USE OF MORE INFORMATION FOR SAFE DRINKING WATER xix
Foreword by Koen Huysman xxi
HOW TO LINK SENSORS WITH YOUR GOALS FOR OPERATIONAL EXCELLENCE xxi
About the Editors xxv
List of Contributors xxvii
Section 1: Background and perspectives 1
Chapter 1: History and perspective – the challenge of the gap between legislation and scientific achievements: The new era of enlightenment 3
1.1 INTRODUCTION 3
1.2 THE HISTORICAL DEVELOPMENT OF CULTURE-BASED METHODS 4
1.3 ADVANTAGES AND LIMITATIONS OF THE CLASSICAL CULTURE-BASED METHODS 5
1.4 MEASURING GENERAL MICROBIAL POPULATIONS OR MICROBIAL INDICATORS 5
1.5 MONITORING FOR COMPLIANCE WITH GUIDELINES VERSUS PROCESS CONTROL 6
1.6 SENSORS 7
1.7 OPPORTUNITIES OFFERED BY SENSORS 7
1.8 NEW INSIGHTS THROUGH MONITORING WITH SENSORS 9
1.9 THE POSITION OF REGULATORS AND UTILITIES 9
1.10 REFERENCES 11
Chapter 2: The need for speed – rapid evolution of microbiological testing in drinking water 15
2.1 INTRODUCTION 15
2.2 ANCIENT AND MEDIEVAL TIMES – EARLY MICROBIOLOGICAL SENSING 16
2.3 19TH CENTURY – LINKING THE WATER CYCLE TO HUMAN HEALTH 18
2.4 20TH CENTURY – ESTABLISHMENT OF REGULATORY FRAMEWORKS 20
2.4.1 Pressure 21
2.4.2 Turbidity 21
2.4.3 Disinfectant residual 21
2.4.4 Faecal indicators 22
2.5 21ST CENTURY – A NEW PARADIGM 22
2.5.1 Aging infrastructure and shifting demand 22
2.5.2 Changing workforce 23
2.5.3 Consumer awareness 23
2.5.4 Evolving science 24
2.6 THE ECONOMICS OF TIME IN DRINKING WATER 24
2.6.1 Water and chemical conservation 25
2.6.2 Time and travel conservation 25
2.6.3 Boil Water advisory mitigation 25
2.6.4 Infrastructure preservation 26
2.7 UPGRADING THE TOOLBOX 26
2.7.1 Online cell counting and sensing 27
2.7.2 Portable and rapid microbiological methods 27
2.7.3 Microorganism identification 28
2.8 THE FUTURE AND THE HOLY GRAIL 29
2.9 CONCLUSION 31
2.10 REFERENCES 32
Chapter 3: Microbiological sensors for drinking water: Terminology and central concepts 35
3.1 INTRODUCTION 35
3.2 MICROORGANISMS 35
3.2.1 Dead or alive? 36
3.2.2 Planktonic or sessile? 37
3.2.3 Pathogenic or harmless? 38
3.3 MICROBIOLOGICAL CONTAMINATION 40
3.3.1 Sources of microbiological contamination in drinking water 40
3.3.2 Location of contamination sources 42
3.3.3 Contamination patterns 43
3.4 MICROBIOLOGICAL SENSORS 44
3.4.1 Goals of monitoring with microbiological sensors 45
3.4.2 Sensor attributes 47
3.4.3 Metrics and measurement principles 49
3.4.4 Monitoring strategies 51
3.5 CONCLUDING REMARKS 52
3.6 REFERENCES 52
Section 2: Industry needs 55
Chapter 4: Water quality monitoring at Danish utilities – current state and needs for the future 57
4.1 INTRODUCTION 57
4.2 OBJECTIVES OF MONITORING WATER QUALITY 58
4.2.1 Legal compliance 58
4.2.1.1 Grab samples 58
4.2.1.2 Online microbiological sensors 59
4.2.2 Detection of contamination 60
4.2.2.1 Severity of contamination 60
4.2.2.2 Source tracking 60
4.2.2.3 Decision support 61
4.2.3 Prevention of contamination 61
4.2.4 Process control and optimisation 61
4.3 CURRENTLY APPLIED WATER QUALITY MONITORING METHODS 61
4.3.1 Standardised methods generally applied by Danish utilities 62
4.3.2 Other commercial methods applied by some Danish utilities 62
4.3.2.1 Commercial sensors applied by utilities 63
4.3.2.2 Additional commercial methods applied by utilities 63
4.4 RESEMBLANCE BETWEEN MONITORING OBJECTIVES AND CURRENTLY APPLIED METHODS 64
4.5 FUTURE NEEDS FOR WATER QUALITY MONITORING 69
4.6 REFERENCES 70
Chapter 5: On-line monitoring of bacteria in drinking water systems: Expected benefits and new challenges for utility operators 73
5.1 INTRODUCTION 73
5.2 EXPECTED BENEFITS 74
5.2.1 On-line monitoring in raw water at Drinking Water Treatment Plant (DWTP) 75
5.2.1.1 Fecal contamination monitoring 75
5.2.1.2 Cyanobacteria-associated bloom monitoring 76
5.2.2 On-line monitoring in Drinking Water Treatment Processes (DWTP) 76
5.2.3 On-line monitoring in Drinking Water Distribution System (DWDS) 77
5.3 CHALLENGES 78
5.3.1 On the track of the ideal sensor 79
5.3.2 Monitoring parameters 79
5.3.3 Validation of measurements 81
5.3.4 Data management 82
5.4 CASE STUDY 83
5.5 CONCLUSION 85
5.6 REFERENCES 86
Section 3: Sensor technologies 89
Chapter 6: Use of fully automated bacterial monitors for enhanced water safety 91
6.1 HISTORICAL DEVELOPMENT OF AUTOMATED BACTERIAL MONITORS BY COLIFAST AS 91
6.1.1 Analysis reagents 92
6.1.2 First automated analyser 92
6.1.3 Flexible on-line analyser 93
6.1.4 Manual Field Kit 95
6.1.5 On-line drinking water analyser 96
6.2 METHODS 98
6.3 APPLICATION EXAMPLES, OPERATION AND INTEGRATION 100
6.3.1 Monitoring raw water quality – Gothenburg, Sweden 100
6.3.2 Process control – treatment step in small WTP 104
6.3.3 Oslo municipality water and wastewater 106
6.3.3.1 Monitoring of drinking water bacterial quality with Colifast ALARM 106
6.3.3.2 Raw water monitoring with CALM 107
6.3.3.3 Environmental monitoring with CALM 108
6.4 BENEFITS AND LIMITATIONS 109
6.5 FUTURE PERSPECTIVES 112
6.6 REFERENCES 113
Chapter 7: Online flow cytometry: Towards a rapid, robust, and reliable microbial sensor 115
7.1 INTRODUCTION: MICROBIAL DYNAMICS MATTER IN AQUATIC ECOSYSTEMS 115
7.2 FCM IS AN AUTOMATABLE MULTIVARIATE MICROBIAL SENSOR 117
7.3 EXPERIENCE GAINED WITH DATA SETS GENERATED DURING THE LAST FIVE YEARS 120
7.3.1 Periodic and aperiodic fluctuations in river water 120
7.3.2 Precipitation-induced contamination of karstic spring water 122
7.3.3 Operationally-induced fluctuations in river bank filtered water 123
7.3.4 Characterising bacterial growth 125
7.4 TOWARDS ROUTINE IMPLEMENTATION 126
7.5 THE NEXT FRONTIERS: HIGH FREQUENCY REAL-TIME FLOW CYTOMETRY (RT-FCM) AND ONLINE VIABILITY ANALYSIS 128
7.6 CONCLUSIONS 130
7.7 REFERENCES 130
Chapter 8: Adenosine Triphosphate (ATP) measurement technology 137
8.1 INTRODUCTION 137
8.2 ATP MEASUREMENT FUNDEMENTALS 138
8.3 FIRST VERSUS SECOND GENERATION ATP MEASUREMENT 140
8.3.1 Overcoming Interferences 140
8.3.2 Extraction and Recovery 141
8.3.3 Importance of Calibration 141
8.3.4 Limit of Detection 142
8.4 METHODS FOR DETECTION OF TOTAL MICROORGANISMS 142
8.4.1 Why Measure Total Microorganisms? 143
8.4.2 Population Specificity 143
8.4.3 Particle Association and Agglomeration 144
8.4.4 Disinfection Efficacy 144
8.5 CASE STUDIES 145
8.5.1 Direct Comparison of Second Generation ATP to Culture-Based Methods 145
8.5.2 Using Second Generation ATP Testing to Optimize Biologically Active Filters 146
8.5.3 The Membrane Biofouling Index 148
8.5.4 Using Second Generation ATP Testing to Address Biological Hotspots 149
8.5.5 Audit of Public Building Water Distribution System 151
8.6 CONCLUSIONS 152
8.7 REFERENCES 153
Chapter 9: Counting totally matters – using grundfos bacmon for network monitoring 155
9.1 INTRODUCTION 155
9.2 TECHNOLOGY AND SOLUTION 157
9.2.1 Mimicking the human brain a bit 157
9.2.2 How does it compare? 158
9.2.3 Air bubbles, biofilm, and fouling taken into account 160
9.3 OVERALL CONCEPT 162
9.4 FROM DUSK TILL DAWN – APPLICATION CASES 163
9.4.1 Flushing filters 165
9.4.2 Network overview and optimization 166
9.4.3 Red alert in food production 168
9.5 FUTURE PERSPECTIVES 169
9.6 REFERENCES 170
Chapter 10: Safety and quality control in drinking water systems by online monitoring of enzymatic activity of faecal indicators and total bacteria 171
10.1 INTRODUCTION 171
10.1.1 The role of monitoring in water safety and quality 171
10.1.2 Rationale for monitoring enzymatic activity 173
10.1.2.1 Total bacteria (‘total activity’) 173
10.1.2.2 Total coliforms and (faecal) indicators 174
10.2 EQUIPMENT DESIGN AND OPERATION 175
10.2.1 Bacterial enzymes 175
10.2.2 Sampling the bacteria and their enzymes 176
10.2.3 Enzymatic reaction 176
10.2.4 Quantifying enzymatic activity 177
10.2.5 Data storage, processing and flow 178
10.2.6 Cleaning 178
10.2.7 Speed: Frequency of sampling and analysis 178
10.2.8 Improvements since the last validation study 178
10.3 RESULTS OF VALIDATION STUDIES 179
10.4 BARCELONA WATER WORKS CASE STUDY: TOTAL BACTERIAL ACTIVITY 179
10.4.1 Description of the treatment plant 179
10.4.1.1 Surface water, the Llobregat River 180
10.4.1.2 Groundwater 180
10.4.2 Monitoring points 182
10.4.2.1 Sand-filtered water 182
10.4.2.2 GAC-filtered water 182
10.4.2.3 Treated water 182
10.4.3 Quality events 182
10.4.4 Verification strategy and methods 183
10.4.5 Results 183
10.4.5.1 Sand filters 184
10.4.5.2 GAC filters 187
10.4.5.3 Treated water 189
10.4.6 Total activity vs. other verification methods 190
10.4.6.1 GAC filtration 190
10.4.6.2 Sand filtration 190
10.4.7 User feedback of BACTcontrol 191
10.5 DISCUSSION 191
10.6 CONCLUSIONS 192
10.7 Acknowledgements 193
10.8 REFERENCES 193
Chapter 11: Mean oxidation state of organic carbon: A novel application to evaluate the extent of oxidation of natural organic matter in drinking water biological treatment 197
11.1 INTRODUCTION 197
11.2 BACKGROUND 198
11.2.1 Quantifying natural organic matter by Photoelectrochemical Chemical Oxygen Demand (peCOD) 198
11.2.2 Mean Oxidation State (MOS) of organic carbon (Cos) 199
11.2.3 Biomass Adenosine Triphosphate (ATP) 200
11.3 MATERIALS AND METHODS 201
11.3.1 Full-scale drinking water biofilters 201
11.3.2 Analytical methods 202
11.3.2.1 Total organic carbon (TOC)/dissolved organic carbon (DOC) 202
11.3.2.2 Photoelectronchemical chemical oxygen demand (peCOD) 202
11.3.2.3 Adenosine triphosphate (ATP) of biomass 202
11.3.2.4 Data analysis 203
11.4 RESULTS AND DISCUSSION 203
11.4.1 Mean oxidation state of organic carbon before/­after biofiltration 203
11.4.2 Evolution of biomass ATP and mean oxidation state of organic carbon 204
11.4.3 Implications for drinking water utilities 207
11.5 CONCLUSION 208
11.6 REFERENCES 208
Section 4: Data collection and interpretation 211
Chapter 12: Test of sensor technologies for monitoring of microbiological drinking water quality 213
12.1 INTRODUCTION 213
12.2 EXPERIMENTAL PROCEDURES 214
12.2.1 Experimental setup 214
12.2.2 Contaminant types 215
12.2.3 Methodology 215
12.2.4 Analyses 217
12.3 RESULTS AND DISCUSSION 218
12.3.1 Specificity and Sensitivity 220
12.3.2 Speed 221
12.3.3 User-friendliness 222
12.3.4 Automatic sampling 224
12.3.5 Characterization of contaminations 225
12.4 CONCLUSIONS 227
12.5 REFERENCES 228
Chapter 13: From sensor to decision – augmented and automated decision-making based on real-time data 231
13.1 INTRODUCTION 231
13.2 CONNECTING SENSORS – LESSONS FROM INTERNET OF THINGS 233
13.2.1 Device-to-Device connection 233
13.2.2 Device-to-Cloud connection 234
13.2.3 Device-to-Gateway connection 235
13.2.4 Cloud-to-Cloud connection 236
13.3 STORAGE STRATEGIES 237
13.4 DATA ANALYTICS 238
13.5 DECISION MAKING – AUGMENTED OR AUTOMATED 240
13.6 REFERENCES 241
Section 5: Water safety, education and legislation 243
Chapter 14: HACCP in drinking water systems 245
14.1 INTRODUCTION 245
14.2 WATER SUPPLY IN DENMARK 246
14.3 HISTORY AND INTERNATIONAL STANDARDS 248
14.4 HACCP – INTRODUCED TO DANISH UTILITIES 249
14.5 HACCP – THE DANISH APPROACH 250
14.5.1 Risk assessment 251
14.5.2 HACCP as a management tool 254
14.5.3 An efficient tool: education and HACCP training programmes 255
14.5.4 Procedures: Drinking water safety brochure 256
14.6 MONITORING OF THE QUALITY OF DRINKING WATER 257
14.7 ON-LINE MICROBIOLOGICAL SENSORS 259
14.8 HANDS-ON EXPERIENCE FROM TWO WATER UTILITIES 259
14.8.1 HACCP and maintenance of pipe systems – an example 260
14.8.2 Case study: Clean water tanks 261
14.8.3 Case study: Truelsbjerg waterworks 262
14.9 HACCP – AN ONGOING PROCESS 263
14.10 CONCLUDING REMARKS 264
14.11 REFERENCES 264
Chapter 15: Enhancing knowledge of microbiological sensors through education 267
15.1 BETTER EDUCATION OF WORK FORCE IN THE WATER SECTOR 267
15.2 Introduction OF MICROBIOLOGICAL SENSORS IN THE EDUCATIONAL SYSTEM 270
15.2.1 Supply engineering education 270
15.2.2 A representative student BSc project on Ice Pigging of drinking water pipes 271
15.2.3 Industry courses and workshops 272
15.2.3.1 Water quality 273
15.2.3.2 Practical hygiene for people working with drinking water 273
15.2.3.3 Practical hygiene for external contractors working for the waterworks 274
15.2.3.4 Ad hoc industry workshops 274
15.3 GAP ANALYSIS AND WAY FORWARD 276
15.4 Acknowledgements 277
15.5 REFERENCES 277
Chapter 16: Challenges of regulatory compliance using sensor technology for drinking water distribution systems 279
16.1 INTRODUCTION 279
16.1.1 USEPA drinking water standards 280
16.1.2 Guidelines for canadian drinking water quality 281
16.1.3 EU drinking water directive 281
16.1.4 World Health Organization (WHO) guidelines 282
16.2 STRATEGIES FOR REGULATORY COMPLIANCE 283
16.2.1 Microbiological contaminants 283
16.2.2 Contaminants specific to water sources 284
16.2.3 Contaminants generated within the water distribution infrastructure 285
16.3 REGULATORY SHORTFALLS 288
16.3.1 Rise of class action lawsuits 290
16.4 ALTERNATE REGULATORY METHODS 291
16.5 ADVANTAGES OF SENSOR TECHNOLOGY FOR REGULATORY COMPLIANCE 293
16.6 THE DATA ANALYTICAL APPROACH FOR MICROBIOLOGICAL SENSORS 295
16.7 CONCLUSIONS 297
16.8 REFERENCES 298
Index 301