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Laboratory and Diagnostic Testing in Ambulatory Care E-Book

Laboratory and Diagnostic Testing in Ambulatory Care E-Book

Marti Garrels

(2018)

Additional Information

Book Details

Abstract

Learn to master the latest lab testing techniques! Ideal for Medical Assisting and Medical Laboratory Technician programs, Laboratory and Diagnostic Testing in Ambulatory Care: A Guide for Healthcare Professionals, 4th Edition, covers the procedures and techniques of commonly used and new CLIA-waived, point-of-care tests, along with some moderately complex tests. Clear, step-by-step instructions and nearly 600 full-color photographs make it easy for you to learn each test and procedure. This edition includes a focus on new technology and the resulting significant advancements in testing.. Expanded coverage of electrocardiography and spirometry reflect the expanding roles of Medical Assistants and Medical Laboratory Technicians. Review questions in the book are accompanied by practice quizzes and videos online to help you master content and hone skills.

  • Comprehensive coverage of the most common CLIA-waived tests prepares you for laboratory testing in the ambulatory setting.
  • A triad organization gives chapters a consistent, easy-to-follow format, with 1) fundamental concepts, 2) step-by-step instructions for CLIA-waived procedures, and 3) advanced concepts help hone your critical-thinking and decision-making skills.
  • Procedure boxes provide step-by-step instructions and full-color photos and illustrations for today’s commonly requested CLIA-waived lab tests.
  • NEW! Content coverage of the latest CLIA-waived and moderately complex testing (including automated CBCs and automated chemistries) and the significant technological advancements
  • NEW! Chapter quizzes online help you ensure comprehension and prepare for classroom and certification exams.
  • UPDATED art program with more than 100 new and updated images that showcase new technology and the lab testing steps and techniques.

Table of Contents

Section Title Page Action Price
Front Cover Cover
Inside Front Cover IFC
Front Matter i
LABORATORY AND DIAGNOSTIC TESTING IN AMBULATORY CARE i
Copyright ii
REVIEWERS iii
PREFACE iv
OUTSTANDING FEATURES iv
ON-LINE RESOURCES: iv
New to This Edition iv
Workbook for Laboratory and Diagnostic Testingin Ambulatory Care (ISBN 9780323532242) iv
TEACH Instructor’s Resources for Laboratory andDiagnostic Testing in Ambulatory Care, located onthe Evolve site at http://evolve.elsevier.com/Garrels/laboratory v
ONLINE LEARNING v
ACKNOWLEDGMENTS vi
Table of Contents vii
PROCEDURES ix
1 Introduction to the laboratory and safety training 1
Abbreviations 3
Objectives 3
Key terms 3
Introduction to the clinical laboratory 3
Purpose of medical laboratories 3
Why laboratory tests are ordered 3
To screen patients for possible disorders. 3
To establish a diagnosis. 3
To monitor the patient’s condition or treatment. 3
Three general ways to analyze specimens 4
Measuring the levels of analytes compared with reference values. 4
Observing and detecting abnormal cells under the microscope. 4
Detecting the presence or absence of an infection. 4
Types of medical laboratories and personnel 4
Reference (or referral) laboratories 4
Reference laboratory professionals 4
Hospital laboratories 6
Ambulatory care settings 7
Advantages of physician’s office laboratory testing. 7
Advantages of reference and hospital laboratories. 7
Physician office laboratory personnel. 7
Attributes of a laboratory professional 7
Laboratory documentation 8
Laboratory requisitions 8
Laboratory reports 10
Laboratory measurements 12
Safety training in the laboratory 13
Biohazard training 13
Cdc and infection control 13
Transmission and the chain of infection. 13
Standard precautions. 13
Hand hygiene. 13
Antimicrobial soap hand wash. 14
Alcohol-based hand rub. 16
Personal protective equipment. 16
Osha biohazard training in bloodborne pathogens standard 16
Diseases caused by bloodborne pathogens. 17
Additional facts regarding the bloodborne viruses 17
Preventive measures to minimize exposure. 17
Procedure after exposure to blood. 20
Documentation of bbps compliance. 21
Chemical hazard training 21
Physical hazard training 23
Laboratory safety evaluation 23
Review questions**Answers to these Review Questions are located in the Appendix on p. 278. 27
Websites 27
2 Regulations, microscope setup, and quality assurance 28
Abbreviations 29
Objectives 29
Key terms 29
Clia: Government regulations 29
Clia levels of complexity and their certification requirements 29
Clia certificate of waiver 29
Clia high and moderately complex laboratories 29
Clia: Provider-performed microscopy procedures certificate 31
Microscope procedure 32
Identifying the parts and functions of a microscope 32
Foundational structures and their functions 32
Illuminating structures and their functions 32
Magnifying structures and their functions 32
Good laboratory practices 36
Quality assurance 36
Quality control 36
Quality control monitoring of qualitative waived tests 37
Quality control monitoring of semiquantitative waived tests 38
Quality control monitoring of quantitative waived tests 38
Optics. 38
External controls. 38
Quality assurance and quality control checklists 40
Hipaa privacy rule 41
Risk management 41
Electronic medical records and bar coding 43
Review questions**Answers to these Review Questions are located in the Appendix on p. 278. 44
Websites 44
3 Urinalysis 45
Objectives 45
Key terms 45
Fundamental concepts and collection procedures 46
Anatomy and physiology of the urinary system 46
Structures of the urinary system 46
Kidneys. 46
Ureters. 46
Urinary bladder. 46
Urethra. 46
Function of the urinary system 46
Formation and flow of urine 48
Filtration. 48
Reabsorption. 48
Secretion. 48
Flow of blood and urine through the urinary system. 48
Composition of urine. 48
Urine specimen collection 48
Random specimen 49
First morning specimen 50
Clean-catch midstream urine specimen 50
Timed urine specimen 50
24-hour collection procedure. 50
Behavioral criteria when working with patients 51
Clia-waived tests 53
Routine urinalysis 53
Physical routine urinalysis 54
Color. 54
Odor. 55
Appearance. 55
Chemical urinalysis 55
Chemical urinalysis—quality assurance. 55
Reagent strip quality control. 56
Automated clinitek method. 57
Urine test strips 57
Specific gravity. 57
Ph. 57
Glucose. 58
Ketones. 58
Bilirubin. 58
Urobilinogen. 58
Blood. 59
Protein. 59
Nitrites. 59
Leukocytes. 59
Microscopic urinalysis 59
Becoming proficient at urinalysis physical and chemical testing and preparing a urine for microscopic testing. 60
Advanced concepts 67
Microscopic elements in urine and their significance 67
Cells (see first row of the atlas—figure 3.14) 67
Red blood cells. 67
White blood cells. 68
Epithelial cells. 68
Casts (see second row of the atlas—figure 3.14) 68
Hyaline casts. 68
Red blood cell casts. 69
White blood cell casts. 69
Renal epithelial cell casts. 69
Granular casts. 69
Waxy casts. 69
Crystals (see third and fourth rows of the atlas) 69
Crystals in acidic urine. 69
Crystals in alkaline urine. 70
Abnormal crystals in acidic urine. 70
Other substances (see fifth row of the atlas) 70
Bacteria. 70
Yeast. 70
Parasites. 70
Sperm. 70
Mucus. 70
Artifacts. 70
Review questions**Answers to these Review Questions are located in the Appendix on p. 278. 71
Websites 71
4 Blood collection 72
Abbreviations 73
Objectives 73
Key terms 73
Fundamental concepts: Theory, safety, and patient preparation 73
Function and structures of blood vessels 73
Types of blood vessels 73
Comparison of blood analytes in capillary and venous blood 75
Most commonly used veins 75
Federal law concerning safety equipment 75
Procedure preparation 76
Proper completion of requisitions 76
Proper identification of the patient 76
Proper patient positioning 76
Blood collection procedures: Capillary puncture and venipuncture 77
Capillary puncture 77
Equipment used in capillary puncture 77
Capillary puncture devices. 77
POCT blood collection devices. 78
Capillary tubes. 78
Plastic pipettes. 79
Microcollection tubes. 79
Order of draw for capillary puncture tubes. 79
Appropriate site selection 79
Site preparation 79
Additional information 80
Newborn screening (formerly pku) 80
Becoming proficient at capillary puncture 82
Venipuncture 82
Equipment used in venipuncture 82
Vacuum collection tubes. 82
Additives and color-coded vacuum tubes. 82
Vacuum tube method using the vacutainer collection system 85
Equipment used in the vacutainer method 85
Vacutainer needle. 85
Vacutainer holder. 85
Tourniquet. 85
Site preparation. 85
Syringe method 86
Equipment used in the syringe method 87
Syringe. 87
Syringe needle. 87
Butterfly needle method 88
Order of draw for all venipuncture methods and other quality assurance considerations 88
Becoming proficient at venipuncture 91
Advanced concepts: Specimen and patient care 108
Preparing blood specimens for laboratory pickup 108
Centrifuge safety 108
Patient issues when locating a suitable vein 108
Obesity 108
Complications during blood collection 109
Hematomas 109
Failure to obtain blood when needle is in the arm 109
Mastectomy 110
Intravenous therapy 110
Edema 111
Areas to avoid 111
Hemolysis 111
Excessive bleeding 111
Neurologic problems 111
Risk management 111
Review questions**Answers to these Review Questions are located in the Appendix on p. 278. 112
Websites 112
5 Hematology 113
Abbreviations 114
Objectives 114
Key terms 114
Fundamental concepts 114
Overview of hematology and blood 114
Red blood cells (erythrocytes) 115
White blood cells 117
Granulocytes. 117
Neutrophils. 117
Eosinophils. 117
Basophils. 117
Nongranulocytes (agranulocytes). 117
Platelets (thrombocytes) 118
Preparing a blood smear to be viewed by the physician or laboratory technician 118
White blood cell identification and differential 120
Red blood cell identification and description 124
Red blood cell atlas. 124
Platelet description 124
Theory of hemostasis 124
Coagulation and testing 124
Summary of fundamental concepts 125
Clia-waived hematology tests 126
Hemoglobin 126
Hemoglobin testing methods 127
Hemocue method 127
I-stat method 127
Hematocrit 127
General spun microhematocrit procedures 127
Hematastat procedure 128
Erythrocyte sedimentation rate 128
False increased rates 128
False decreased rates 128
Other interferences 128
Sediplast ESR system 128
Streck 30-minute manual ESR system 129
Prothrombin time 129
Becoming proficient at clia-waived hematology tests 129
Advanced concepts 139
Complete blood count 139
Abnormal complete blood count findings 140
Anemias 141
White blood cell disorders 141
Clia-nonwaived (moderately complex) automated hematology systems 142
Qbc star centrifugal hematology system 142
Quality assurance 143
Review questions**Answers to these Review Questions are located in the Appendix on p. 278. 143
6 Chemistry 144
Abbreviations 145
Objectives 145
Key terms 145
Fundamental concepts 145
Blood plasma 145
Blood chemistry specimens 146
Reference laboratory specimens for blood chemistry testing 146
Physician’s office laboratory specimens for clia-waived chemistry tests 147
Glucose and lipid metabolism 149
Glucose metabolism 149
Diabetes mellitus 150
Insulin-dependent diabetes mellitus (type 1). 150
Non–insulin-dependent diabetes mellitus (type 2). 150
Prediabetes. 151
Gestational diabetes. 151
Transient diabetes. 151
Blood glucose screening and monitoring tests 151
Fasting blood glucose. 151
Oral glucose tolerance test or 2-hour postprandial blood sugar. 151
Random glucose test. 152
Glucose tolerance test. 152
Glycosylated hemoglobin, glycated hemoglobin, hgb a1c , or “a1c”. 152
Lipid metabolism and testing 153
Lipid metabolism 153
Cholesterol. 153
Lipoproteins. 153
Total cholesterol/high-density lipoprotein ratio. 154
Triglycerides. 154
The lipid panel 154
Clia-waived chemistry tests 154
Principle of photometers and spectrophotometers 154
Quality assurance when using optical instruments 154
Westgard’s rules for monitoring quality control results 155
Clia-waived glucose tests 155
Glucose monitoring devices 155
Glycosylated hemoglobin (a1c) 156
Cholesterol and lipid profiles with the cholestech ldx 156
Liver enzyme testing with the cholestech ldx 156
I-stat chem8 156
Fecal occult blood testing—three methods 156
Occult blood testing with the guaiac slide method 157
Colocare method using test pads 157
Ifob (immunochemical fecal occult blood) method (or “fit” fecal immunochemical test) 157
Summary of clia-waived tests 158
Becoming proficient at clia-waived chemistry testing 158
Advanced concepts 173
I-stat 173
Piccolo xpress 173
Metabolic panels: Basic and comprehensive 173
Kidney or renal panel 174
Electrolyte panel 174
Liver or hepatic panel 175
Cardiac panel 175
Thyroid panel 175
Individual analytes and their disease associations 175
Summary 178
Review questions**Answers to these Review Questions are located in the Appendix on p. 278. 178
Websites 179
7 Immunology 180
Objectives 180
Key terms 180
Fundamental concepts 181
Overview of immunology 181
The immune process 181
First line of defense: Natural barriers. 182
Second line of defense: Nonspecific internal response. 182
Third line of defense: Acquired (adaptive) immunity. 182
Cell-mediated immunity. 182
Humoral immunity (antibody-mediated immunity). 183
Classes of antibodies (immunoglobulins). 184
Ways to acquire specific immunity. 184
Two types of allergy testing 184
In vivo testing 184
In vitro testing 184
Clia-waived immunology tests 185
Clia-waived enzyme-linked immunoassays 185
Pregnancy testing 185
Mononucleosis testing 186
Helicobacter pylori testing 187
Human immunodeficiency virus 187
Advanced concepts 198
Agglutination reactions (non–clia-waived tests) 198
Immunohematology 198
Abo blood typing 198
Rh system 198
Hemolytic disease of the newborn and rhogam. 199
Enzyme-linked immunosorbent assay: Quantitative analysis 199
Antibody titers 199
Summary of immunologic tests 200
Review questions**Answers to these Review Questions are located in the Appendix on p. 279. 201
Websites 201
8 Microbiology 202
Objectives 202
Key terms 202
Fundamental concepts 203
Overview of microbiology 203
Classification of microorganisms 203
Viruses. 203
Bacteria. 203
Fungi. 203
Parasites. 203
Nomenclature of microorganisms 203
Structural characteristics of bacteria 203
Coccus. 204
Bacillus. 205
Spirillum. 205
Other bacterial structures. 205
Collecting, transporting, and processing microbiology specimens 205
Collecting a throat specimen 206
Collecting a nasal specimen 206
Collecting a blood culture specimen 206
Collecting a urine culture specimen 207
Collecting a chlamydia specimen 207
Collecting a gonorrhea specimen 207
Collecting a fecal specimen for ova, parasites, and bacteria 208
Transporting specimens by mail 208
Microbiology smears, stains, and wet mounts 209
Gram stain 209
Smear preparation 209
Acid-fast stains 210
Wet mounts 211
Koh preparation 211
Pinworm specimen collection and microscopic results 211
Clia-waived microbiology tests 215
Streptococcus group a testing 215
Rapid strep testing (clia waived) 216
Bacitracin method 216
Influenza 216
Influenza testing (clia waved) 216
Advanced concepts 221
Growth requirements of bacteria 221
Oxygen requirements 221
Nutrient requirements 221
Media used for growing bacteria 221
Microbiology equipment 222
Equipment used to inoculate culture plates 222
Equipment used for incineration 222
Culturing methods 222
Inoculation of media 222
Sensitivity testing 223
Urine culture 223
Pathogenic organisms seen frequently in physician office laboratories 224
Pathogenic bacteria 224
Pathogenic fungi 224
Parasites and protozoa 224
Emerging infectious diseases 224
Bioterrorism 224
Agents used in viral bioterrorism 224
Smallpox. 224
Viral hemorrhagic fever. 224
Biological toxin used in bioterrorism 224
Botulism. 224
Agents used in bacterial bioterrorism 225
Plague. 225
Tularemia. 225
Anthrax. 226
Reference tables for common infectious diseases categorized 226
Review questions**Answers to these Review Questions are located in the Appendix on p. 279. 231
Websites 232
9 Toxicology 233
Objectives 233
Key terms 233
Fundamental concepts 233
Overview of toxicology 233
Drugs of abuse 234
Clia-waived drug screening tests 236
Advanced concepts 240
Therapeutic drug monitoring 240
Common therapeutic drugs that are tested for toxicity 240
Pharmacokinetics 240
Liberation 240
Absorption and distribution 240
Metabolism and elimination 240
Drug half-life and specimen collection 241
Other toxicology tests 241
Summary 241
Review questions**Answers to these Review Questions are located in the Appendix on p. 279. 241
True or false 241
Fill in the blanks 241
Multiple choice 241
Websites 241
10 Electrocardiography 243
Abbreviations 244
Objectives 244
Key terms 244
Fundamental concepts 244
Anatomy of the heart 244
Chambers and vessels of the heart 244
Cardiac cycle and electrical conduction system in the heart 244
Brief history of the electrocardiogram 245
Modern electrocardiography monitors and tracings 245
Electrodes 246
Limb lead wires 246
Standard limb leads. 248
Augmented limb leads. 248
Precordial chest lead wires 248
Standardization and interpretation of the electrocardiograph 248
Standardization. 248
Interpretation of electrocardiogram. 249
Diagnostic procedures: Electrocardiography 250
Electrocardiography patient preparations, instructions, and identification of artifacts 250
Patient preparation 250
Identifying and correcting electrocardiography artifacts 250
Advanced concepts 257
Ambulatory electrocardiography monitoring 257
Zio patch wireless electrocardiography monitoring 257
Cardiac arrhythmias and the electrocardiogram 258
Acute myocardial infarction 258
Atlas of atrial arrhythmias 258
Atlas of ventricular arrhythmias 258
Becoming proficient at electrocardiography tests. 262
Review questions**Answers to these Review Questions are located in the Appendix on p. 279. 263
Website 263
11 Spirometry 264
Abbreviations 264
Objectives 264
Key terms 264
Fundamental spirometry concepts 264
Anatomy of the lungs 265
Modern spirometry testing and tracings 265
Volume/time chart 267
Flow/volume chart—”peak flow chart” 267
Predicted values and percentiles 267
Quality control–spirometer calibration 267
Diagnostic procedures: Spirometry 267
Spirometer patient preparations, instructions, and identification of artifacts 267
Patient preparation 267
Coaching the maneuver 267
Postbronchodilator response 268
Advanced spirometry concepts 270
Peak expiratory flow screening and monitoring 270
Asthma therapies: Inhalers, nebulizers, and oxygen 271
Nebulizer procedure 272
Administering oxygen by nasal cannula 273
Becoming proficient at spirometry diagnostic tests 273
Review questions**Answers to these Review Questions are located in the Appendix on p. 279. 277
Websites 277
APPENDIX A Answers to Review Questions 278
CHAPTER 1 278
CHAPTER 2 278
CHAPTER 3 278
CHAPTER 4 278
CHAPTER 5 278
CHAPTER 6 278
CHAPTER 7 279
CHAPTER 8 279
CHAPTER 9 279
CHAPTER 10 279
CHAPTER 11 279
APPENDIX B Herb/Laboratory Test Interactions 280
APPENDIX C Common Laboratory and Diagnostic Tests 283
BLOOD ANALYSIS 283
Complete Blood Count 283
Red Blood Cell Count 283
Hemoglobin Count 283
Hematocrit Count 283
White Blood Cell Count 283
Differential White Blood Cell (Leukocyte) Count 284
Platelet (Thrombocyte) Count 284
BLOOD CHEMISTRIES 284
Chemistries 284
Albumin 284
Alkaline Phosphatase 284
Aspartate Aminotransferase (Serum Glutamic Oxaloacetic Transaminase) 285
Bilirubin 285
Creatinine 285
Lactate Dehydrogenase 285
Total Protein 285
Urea Nitrogen/Blood Urea Nitrogen 285
Uric Acid 285
Thyroid Function Tests 286
Thyroxine 286
Triiodothyronine 286
Thyroid-Stimulating Hormone 286
Lipid Profile 286
Total Cholesterol 286
Triglycerides 286
High-Density Lipoprotein Cholesterol 286
Low-Density Lipoprotein Cholesterol 287
Electrolytes 287
Chloride 287
Potassium 287
Sodium 287
Carbon Dioxide 287
Calcium 287
Ionized Calcium 288
Magnesium 288
Phosphorus 288
CLOTTING AND COAGULATION STUDIES 288
Partial Thromboplastin Time 288
Prothrombin Time 288
Critical Value 288
Bleeding Times 288
Erythrocyte Sedimentation Rate 288
GLUCOSE MONITORING 289
Glucose Tolerance Test 289
Fasting Blood Glucose Levels 289
Two-Hour Postprandial 289
Glycosylated Hemoglobin/Glycohemoglobin 289
TOXICOLOGY STUDIES AND DRUG SCREENS 289
BLOOD SCREENING TESTS 290
Drug Levels 290
Digoxin 290
Digitoxin 290
Theophylline 290
Lidocaine 290
Lithium 290
Prograf (Tacrolimus)/FK-506 290
Phenytoin (Dilantin) 290
Alcohol Levels 290
CARDIAC ENZYMES/CARDIAC ISOENZYMES 290
C-Reactive Protein 290
Creatine Kinase 291
Creatine Kinase Isoenzymes 291
Lactate Dehydrogenase 291
Aspartate Aminotransferase (Serum Glutamic Oxaloacetic Transaminase) 291
Alanine Aminotransferase (Serum Glutamic Pyruvate Transaminase) 291
URINE STUDIES 291
Urinalysis/Clean Catch Urinalysis 291
Culture and Sensitivity of Urine 292
CARDIOLOGY TESTS 292
Electrocardiogram (12 Lead) 292
Echocardiogram 292
Cardiac Stress Echocardiogram 292
Holter Monitor 292
Cardiac Event Recorders 292
Multigated Blood Pool Study Scan 293
Cardiac Stress Test (Cardiac Perfusion Scan) 293
Exercise Tolerance Test 293
Hepatobiliary (Gallbladder) Scan with Ejection Fraction 293
Pulse Oximeter 293
Cardiac Catheterization 293
IMAGING STUDIES 293
Radiographs 293
Magnetic Resonance Imaging/Magnetic Resonance Angiogram Scan 294
Computerized Tomography Scan 294
Fluoroscopy 294
Sonogram, Ultrasound, and Echogram 294
Myelogram 294
Positron Emission Tomography Scan 294
STOOL ANALYSIS 295
Hemoccult/Guaiac Test 295
Ova, Larva, and Parasite Tests 295
ENDOSCOPY TESTS 295
Endoscopy 295
Esophagogastroduodenoscopy/Upper Endoscopy 295
Esophagoscopy 295
Gastroscopy 295
Colonoscopy 295
Sigmoidoscopy 295
Proctoscopy 295
Cystoscopy 295
Ureteroscopy 295
Bronchoscopy 296
Arthroscopy 296
ARTERIAL BLOOD GASES 296
Arterial Blood Gas Analysis 296
PULMONARY FUNCTION STUDIES 296
Pulse Oximeter 296
Peak Flow 296
Spirometry 296
Methacholine Challenge 297
Sputum Studies 297
Pulmonary Function 297
MISCELLANEOUS TESTS 297
Culture and Sensitivity Studies 297
Bone Marrow Studies 297
Immune and Immunoglobulin Studies 297
Biopsies 297
Lumbar Puncture 297
Electroencephalogram 298
Electromyelography 298
Gastric Analysis 298
Human Chorionic Gonadotropin 298
SCREENING 298
Tuberculosis Screening (Mantoux) 298
Cancer or Tumor Markers as Screening Tools 298
Prostate-Specific Antigen 298
CA 125 299
CA 19-9 299
CA 15-3 299
Carcinoembryonic Antigen 299
Alpha-Fetoprotein 299
Papanicolaou (Pap) Smear 299
Class System 299
Bethesda System 299
Mammogram 300
INDEX 301
A 301
B 301
C 302
D 303
E 303
F 303
G 304
H 304
I 305
J 305
K 305
L 305
M 306
N 306
O 306
P 307
Q 308
R 308
S 308
T 309
U 309
V 309
W 310
Y 310
Z 310