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Book Details
Abstract
Activated Sludge Separation Problems: Theory, Control Measures, Practical Experiences, Second Edition, describes the most common activated sludge separation problems and explains the main reasons for the growth of the different filamentous microorganisms in activated sludge. The book summarizes the identification techniques for important groups of activated sludge microorganisms both based on conventional microscopic analysis and using the biological molecular tools available today (FISH and PCR).
This new edition, with 70% new and updated material, also provides explanation of basic activated sludge process principles and of parameters necessary for process control and operation. The theory of secondary clarifies is described to the extent necessary for understanding the construction and operation of secondary clarifiers. The activated sludge reactor and secondary clarifies are treated as one system and the interactions are explained. The wide range of experiences around the world is documented and the methods to avoid the proliferation of these organisms are presented and critically reviewed.
Activated Sludge Separation Problems consists of six chapters, presenting up-to-date technical and scientific aspects of these processes. The new edition also features an extended list of literature references for further reading.
The book will be a valuable help for students of environmental engineering, wastewater specialists, plant operators and designers of activated sludge plants. It is also useful for specialists in wastewater operation laboratories, especially for those studying activated sludge separation properties.
Table of Contents
Section Title | Page | Action | Price |
---|---|---|---|
Cover | Cover | ||
Contents | vii | ||
Abbreviations | xiii | ||
List of Contributors | xvii | ||
Preface | xxi | ||
Chapter 1: Wastewater characterization | 1 | ||
1.1 INTRODUCTION | 1 | ||
1.2 GROSS PARAMETERS | 2 | ||
1.3 PHYSICAL PROPERTIES | 4 | ||
1.4 ORGANIC MATTER | 5 | ||
1.5 INORGANIC MATTER | 7 | ||
1.6 MICROPOLLUTANTS | 8 | ||
1.7 BIODEGRADABILITY | 11 | ||
1.7.1 COD, Nitrogen and phosphorus fractionation | 11 | ||
1.7.2 Evaluation methods | 14 | ||
1.7.2.1 Carbonaceous substrate (COD) | 14 | ||
1.7.2.2 Nitrogen and phosphorus | 16 | ||
1.7.2.3 Micropollutants | 16 | ||
REFERENCES | 17 | ||
Chapter 2: The microbiology of the activated sludge process | 21 | ||
2.1 INTRODUCTION | 21 | ||
2.2 MICROORGANISMS IN ACTIVATED SLUDGE | 22 | ||
2.2.1 Bacteria: cell structure | 22 | ||
2.2.2 Inclusions | 24 | ||
2.3 THE IDENTIFICATION OF BACTERIA | 24 | ||
2.3.1 Conventional taxonomy | 25 | ||
2.3.2 Molecular taxonomy | 26 | ||
2.3.3 Molecular characterization of mixed biomass | 26 | ||
2.3.3.1 Polymerase Chain Reaction (PCR) | 27 | ||
2.3.3.2 Fluorescence In Situ Hybridization (FISH) | 27 | ||
2.4 FILAMENTOUS BACTERIA | 28 | ||
2.5 MICROTHRIX PARVICELLA | 36 | ||
2.5.1 Identification of M. parvicella | 38 | ||
2.5.2 Physiology of ‘Candidatus M. parvicella’ | 38 | ||
2.6 THIOTHRIX | 39 | ||
2.7 NOSTOCOIDA LIMICOLA | 40 | ||
2.8 POLYPHOSPHATE ACCUMULATING ORGANISMS (PAO) | 40 | ||
2.9 GLYCOGEN ACCUMULATING ORGANISMS (GAO) | 41 | ||
2.10 NITRIFIERS | 42 | ||
2.11 DENITRIFIERS | 42 | ||
REFERENCES | 43 | ||
Chapter 3: Activated sludge separation problems | 53 | ||
3.1 INTRODUCTION | 53 | ||
3.2 ‘WELL-SETTLING’ ACTIVATED SLUDGE | 54 | ||
3.2.1 Requirements | 54 | ||
3.2.1.1 Measurement of settling and thickening properties | 55 | ||
3.2.2 Microscopic features of well settling activated sludge | 57 | ||
3.3 ACTIVATED SLUDGE SEPARATION PROBLEMS | 57 | ||
3.3.1 Poor floc microstructure | 58 | ||
3.3.1.1 Dispersed growth | 58 | ||
3.3.1.2 Unsettleable microflocs | 59 | ||
3.3.1.3 Viscous bulking | 59 | ||
3.3.2 Poor floc macrostructure | 61 | ||
3.3.2.1 Filamentous bulking | 61 | ||
3.3.2.2 Foaming caused by filamentous microorganisms | 62 | ||
3.3.3 Other reasons | 64 | ||
3.3.3.1 Rising sludge | 64 | ||
3.4 SUMMARY | 65 | ||
ACKNOWLEDGMENT | 65 | ||
REFERENCES | 65 | ||
Chapter 4: Aeration tank and secondary clarifier as one system | 67 | ||
4.1 INTRODUCTION | 67 | ||
4.2 AERATION TANK AND SECONDARY CLARIFIER INTERACTIONS | 68 | ||
4.2.1 Activated sludge process | 68 | ||
4.2.2 Secondary clarifier | 69 | ||
4.2.2.1 General performance, thickening function | 69 | ||
4.2.2.1.1 Solids flow theory | 70 | ||
4.2.2.1.2 State point analysis | 72 | ||
4.2.3 Separation function | 76 | ||
4.2.4 BOD5 | 76 | ||
4.2.5 COD | 77 | ||
4.2.6 Nitrogen and phosphorus | 77 | ||
4.3 THE IMPACT OF AERATION BASIN EQUIPMENT AND OPERATION ON THE PERFORMANCE OF SECONDARY CLARIFIERS | 77 | ||
4.3.1 Mechanical vs. diffused-air aeration | 77 | ||
4.3.2 Mixed liquor mixing | 78 | ||
4.3.3 Degasification and reflocculation in aeration tanks | 79 | ||
4.4 FEATURES OF SECONDARY CLARIFIER CONSTRUCTION | 80 | ||
4.4.1 Inlet structure with a flocculation zone | 81 | ||
4.4.1.1 Principles of a flocculation zone | 81 | ||
4.4.1.2 Conventional flocculation zones with tangential flow regime | 82 | ||
4.4.1.3 Novel flocculation zone with deflectors and a vertical flow regime | 83 | ||
4.4.1.4 Flocculation zone with deflectors with variable profile | 83 | ||
4.4.2 Outlet structure | 84 | ||
4.4.2.1 Peripheral vs. internal effluent launders | 84 | ||
4.4.2.1.1 Circular clarifier, peripheral effluent launder (with inboard weir) | 84 | ||
4.4.2.1.2 Circular clarifier, internal effluent launder (with inset double-sided weirs) | 85 | ||
4.4.2.1.3 Effluent launders in rectangular clarifiers with longitudinal flow | 86 | ||
4.4.3 Scum baffles | 86 | ||
4.5 EFFICIENT SCUM REMOVAL FROM THE SURFACE OF SECONDARY CLARIFIERS | 87 | ||
4.5.1 Conventional scum boxes | 87 | ||
4.5.2 ‘Travelling’ scum boxes | 88 | ||
4.5.3 Pneumatic systems | 88 | ||
4.6 REMOVAL OF SETTLED AND THICKENED SLUDGE | 90 | ||
4.6.1 Effect on the final effluent quality | 90 | ||
4.6.2 Mechanical scrapers | 90 | ||
4.6.3 Vacuum sludge removal | 91 | ||
4.7 OPERATION OF AERATION TANK – SECONDARY CLARIFIER SYSTEM FOR BULKING AND FOAMING CONTROL | 91 | ||
4.7.1 Use of chemicals in activated sludge process | 92 | ||
4.7.1.1 Use of iron salts | 93 | ||
4.7.1.2 Use of aluminium salts | 93 | ||
4.7.1.3 Oxidizing agents | 94 | ||
4.7.1.4 Use of organic flocculants | 95 | ||
4.7.2 Operation of secondary clarifiers | 95 | ||
4.7.2.1 Automatic sludge blanket level detector | 95 | ||
4.7.2.2 Suspended Solids Sensors | 96 | ||
REFERENCES | 96 | ||
Chapter 5: Bulking and foaming control methods | 99 | ||
5.1 INTRODUCTION | 99 | ||
5.1.1 Microscopic characterization of the activated sludge | 100 | ||
5.1.2 Biological foam | 101 | ||
5.1.3 Bulking | 103 | ||
5.1.4 The problem of excess sludge production and its disposal | 103 | ||
5.2 SPECIFIC CONTROL METHODS | 105 | ||
5.2.1 Bulking due to low (F/M) ratio | 105 | ||
5.2.1.1 Continuous plug-flow reactors | 107 | ||
5.2.1.2 Selectors | 109 | ||
(a) Design based on a biokinetic models | 109 | ||
(b) Design based on empirical criteria | 110 | ||
(c) Enhancing the selector effect: anoxic and anaerobic selectors | 111 | ||
5.2.1.3 Sequencing batch reactors | 112 | ||
5.2.1.4 Role of storage phenomena in microbial competition in substrate gradient processes | 112 | ||
5.2.2 Bulking due to low dissolved oxygen concentrations | 117 | ||
5.2.3 Bulking due to low nutrient concentration | 118 | ||
5.2.4 Bulking due to fatty acids in the influent stream: control methods for Microthrix parvicella | 120 | ||
5.2.5 Microbial and enzymatic preparations | 121 | ||
5.3 NON-SPECIFIC CONTROL METHODS | 122 | ||
5.3.1 Oxidizing agents | 122 | ||
5.3.2 Weighting or flocculating agents | 126 | ||
5.3.3 Specific biocide | 127 | ||
5.4 CONTROL OF NON-FILAMENTOUS BULKING (‘VISCOUS BULKING’) | 127 | ||
5.5 AVOIDING POOR SETTLING PROPERTIES: ALTERNATIVE SEPARATIONS OF ACTIVATED SLUDGE | 128 | ||
REFERENCES | 129 | ||
Chapter 6: Experiences in various countries | 139 | ||
6.1 INTRODUCTION | 139 | ||
6.2 AUSTRALIA | 140 | ||
6.2.1 General situation | 140 | ||
6.2.2 What do we know of these filamentous bacteria? | 142 | ||
6.2.3 Bulking and foaming filamentous bacteria in Australian activated- sludge plants | 143 | ||
6.2.3.1 The main foaming organisms | 144 | ||
6.2.3.2 The main bulking filamentous bacteria | 145 | ||
6.2.4 Do filamentous bacteria populations in the same treatment plant change over time and can we control them? | 147 | ||
6.2.4.1 Case Study – Excessive biological foam accumulation trouble shooting | 148 | ||
6.2.4.2 Summary of case study | 150 | ||
6.2.5 The future | 151 | ||
6.3 AUSTRIA | 151 | ||
6.3.1 Intention of the investigation | 151 | ||
6.3.2 Organization of the assessment | 152 | ||
6.3.2.1 General | 152 | ||
6.3.2.2 Carrying out the investigation | 152 | ||
6.3.2.3 Questionnaire | 153 | ||
6.3.2.4 Participation of WWTPs | 154 | ||
6.3.2.5 Categorization of plants and sludges | 154 | ||
6.3.3 Methodology | 155 | ||
6.3.3.1 Microscopy | 155 | ||
6.3.3.2 Parameters for analysis | 155 | ||
6.3.3.3 Data plausibility | 156 | ||
6.3.3.4 Data processing and statistical analysis | 156 | ||
6.3.4 Results | 157 | ||
6.3.4.1 F/M ratio of the treatment plants | 157 | ||
6.3.4.2 Sludge volume index (SVI) | 158 | ||
6.3.4.3 Sludge volume index and plant size | 159 | ||
6.3.4.4 Occurrence of filament types | 159 | ||
6.3.4.5 Seasonal appearance of bulking sludge | 162 | ||
6.3.4.6 F/M ratio and SVI | 162 | ||
6.3.4.7 Influence of filament types on SVI | 163 | ||
6.3.5 Summary and conclusion | 164 | ||
6.4 BELGIUM | 165 | ||
6.4.1 General situation | 165 | ||
6.4.2 The M. parvicella problem | 166 | ||
6.4.2.1 The filamentous types in the activated sludge | 166 | ||
6.4.2.2 The filamentous types in the foam | 168 | ||
6.4.2.3 Conclusion | 168 | ||
6.4.3 Polyaluminium chloride to tackle M. parvicella | 168 | ||
6.4.3.1 Curative approach | 168 | ||
6.4.3.2 Preventive approach | 170 | ||
6.4.3.3 Conclusions | 171 | ||
6.4.4 Microthrix parvicella monitoring: a revised methodology | 171 | ||
6.4.4.1 The MICPARV method | 171 | ||
6.4.4.2 The real-time PCR method | 172 | ||
6.4.4.3 Novel methods in practice | 173 | ||
6.4.5 Conclusion | 174 | ||
6.5 CHINA | 174 | ||
6.5.1 Introduction | 174 | ||
6.5.2 Distribution of filamentous bacteria in activated sludge | 176 | ||
6.5.3 Studies on sludge bulking processes and control strategy | 177 | ||
6.5.3.1 Wastewater treatment performance and population dynamics over a sludge bulking cycle | 177 | ||
6.5.3.2 Control strategy for M. parvicella overgrowth | 179 | ||
6.6 CZECH REPUBLIK | 180 | ||
6.6.1 Separation problems – situation up to the 1980s | 180 | ||
6.6.2 Separation problems – situation up to the mid-1990s | 181 | ||
6.6.3 WWTPs screening – 1995–2000 | 181 | ||
6.6.3.1 Methodology | 181 | ||
6.6.3.2 Microscopic analysis and sedimentation properties evaluation | 182 | ||
6.6.4 Results of separation problems and filamentous micro-organisms screening | 182 | ||
6.6.4.1 Filamentous microorganism abundance | 183 | ||
6.6.4.2 Dominant filamentous microorganisms in sludge and foam | 183 | ||
6.6.5 Development of filamentous population in Czech activated sludge plants between 1997 and 1998 | 184 | ||
6.6.5.1 SVI and total abundance of filaments | 184 | ||
6.6.5.2 Dominant filamentous microorganisms in activated sludge mixed liquor and foams | 185 | ||
6.6.6 Screening of eight nutrient removal plants in 2000 | 187 | ||
6.6.6.1 Characterization of monitored WWTPs | 187 | ||
6.6.6.2 Activated sludges and settling properties | 188 | ||
6.6.6.3 Dominant filamentous microorganisms in activated sludge mixed liquor | 188 | ||
6.6.6.4 Biological foams | 189 | ||
6.6.7 FOAM CONTROL STRATEGIES | 189 | ||
6.6.7.1 Water sprays | 189 | ||
6.6.7.2 Skimming (mechanical removal) of foam | 190 | ||
6.6.7.3 Return activated sludge manipulation | 190 | ||
6.6.7.4 Manipulation of SRT | 191 | ||
6.6.7.5 Additional installation of baffles | 191 | ||
6.6.7.6 Initial contact zones (‘selectors’) | 192 | ||
6.6.7.7 Chlorination of foam | 192 | ||
6.6.8 Development in the last decade | 193 | ||
6.6.8.1 Activated sludge bulking | 194 | ||
6.6.8.2 Activated sludge foaming | 195 | ||
6.7 DENMARK | 197 | ||
6.7.1 General situation | 197 | ||
6.7.2 MiDAS: Large-scale survey of the microbiology of Danish WWTPs | 198 | ||
6.7.3 Wastewater and WWTP characteristics | 199 | ||
6.7.4 Settling properties in Danish nutrient removal plants | 199 | ||
6.7.5 Filamentous community composition | 202 | ||
6.7.5.1 q-FISH based survey of Danish WWTPs (2008–2012) | 202 | ||
6.7.5.2 16S rRNA gene amplicon sequencing-based survey of Danish WWTPs (2006–2014) | 204 | ||
6.7.6 The future: surveillance and control by DNA analyses | 208 | ||
6.8 FRANCE | 209 | ||
6.8.1 General situation | 209 | ||
6.8.1.1 Bulking and foaming situation before 2005 | 210 | ||
6.8.2 Current settling and foaming problems and control measures (2012 survey) | 211 | ||
6.8.2.1 Characteristics of the surveyed WWTPs | 212 | ||
6.8.2.2 Occurrence of settling and/or foaming problems | 213 | ||
6.8.2.3 Factors influencing settling and/or foaming problems | 214 | ||
6.8.2.4 Correlation between settling/foaming problems and the process configuration of the surveyed WWTPs | 214 | ||
6.8.2.5 Control measures | 215 | ||
6.8.3 A Case study: metallic salt addition in an industrial size pilot-plant subject to M. parvicella bulking and foaming | 215 | ||
6.8.4 Conclusions | 217 | ||
6.8.5 Acknowledgments | 218 | ||
6.9 GREECE | 218 | ||
6.9.1 General situation | 218 | ||
6.9.2 Reason for dysfunctions and filamentous bacteria identified | 218 | ||
6.9.3 Solution adopted | 221 | ||
6.9.4 A Greek case study | 222 | ||
6.9.4.1 Settling and foaming problems | 222 | ||
6.9.4.2 Setting up and applying a control strategy | 222 | ||
6.10 ITALY | 225 | ||
6.10.1 General situation | 225 | ||
6.10.2 Characterizing the activated sludge and the qualification circuit | 225 | ||
6.10.3 Filament surveys | 226 | ||
6.10.4 Control methods | 227 | ||
6.10.4.1 Non-specific methods | 228 | ||
6.10.4.2 Specific methods | 228 | ||
6.10.4.3 Case studies with specific solutions | 228 | ||
6.11 MALAYSIA | 233 | ||
6.11.1 General situation | 233 | ||
6.11.2 Causes of plant dysfunction | 235 | ||
6.11.3 Implemented control strategies of filamentous sludge bulking in Malaysia | 237 | ||
6.11.4 Future scenario of sludge bulking occurrences in Malaysia | 238 | ||
6.12 SOUTH AFRICA | 239 | ||
6.12.1 General situation | 239 | ||
6.12.2 Overview of filamentous bulking and foaming in South African wastewater treatment works | 241 | ||
6.12.3 Case study | 241 | ||
6.12.3.1 Filamentous bacteria and sludge bulking in seven South African municipal works | 241 | ||
6.12.3.2 Filamentous bacteria abundance and floc structures | 241 | ||
6.12.3.3 Gauteng Province | 245 | ||
6.12.3.4 KwaZulu-Natal Province | 245 | ||
6.12.3.5 Western Cape Province | 246 | ||
6.12.3.6 Prevalence of Type 0092 in South African biological nutrient removal works | 247 | ||
6.12.4 Remedial methods to control filamentous bulking and foaming in South Africa | 247 | ||
6.12.4.1 Physical and chemical treatment methods | 247 | ||
6.12.4.2 The effect of anoxic–aerobic selectors on filamentous bacteria | 248 | ||
6.13 SPAIN | 248 | ||
6.13.1 General situation | 248 | ||
6.13.1.1 Wastewater composition | 249 | ||
6.13.1.2 Treatment level | 249 | ||
6.13.1.3 Level of application of microbiological control: interlaboratory exercises | 251 | ||
6.13.2 Separation problems and control methods applied | 252 | ||
6.13.2.1 Major filaments responsible for problems in Spanish Activated Sludge Plants | 252 | ||
6.13.2.2 Other solid separation problems | 253 | ||
6.13.2.3 Reasons for dysfunctions in Spanish activated sludge plants | 255 | ||
6.13.2.4 Control measures applied | 256 | ||
6.13.3 Spain case study | 257 | ||
6.13.3.1 Viscous bulking with Nocardioforms and Type 021N growth | 258 | ||
6.13.3.2 Filamentous bulking caused by Sphaerotilus natans | 261 | ||
6.13.4 Acknowledgement | 263 | ||
6.14 USA | 263 | ||
6.14.1 General situations | 263 | ||
6.14.2 Control of filaments in activated sludge systems | 264 | ||
6.14.3 Other solid separation problems | 270 | ||
6.14.4 Foaming | 270 | ||
6.14.5 Viscous bulking and dispersed growth | 271 | ||
6.14.6 Conclusions | 275 | ||
REFERENCES | 275 | ||
Index | 293 |