BOOK
Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures
(2015)
Additional Information
Book Details
Abstract
Molecular techniques are emerging as a rapid and powerful approach for microorganism detection. This project reviewed the efficacy of PCR assays for Cryptosporidium, Microcystis, adenovirus and ammonia oxidising bacteria, as well as candidate techniques for DNA extraction and inhibitor removal. The literature review led to an evaluation of DNA extraction kits and reagents for PCR; finalisation of assay formats; the development of PCR controls and Standard Operating Procedures (SOP); analysis of assay robustness using real samples; and completion of an Inter-laboratory trial using the SOP’s developed.
This book is co-published with Water Research Australia.
Table of Contents
| Section Title | Page | Action | Price |
|---|---|---|---|
| Cover | Cover | ||
| CONTENTS | 4 | ||
| 1. PCR DETECTION OF CRYPTOSPORIDIUM | 5 | ||
| Preface and Foreword | 5 | ||
| Health and Safety Information | 5 | ||
| Hazard Warnings | 5 | ||
| Hazard Risk Assessment | 6 | ||
| Performance Characteristics | 6 | ||
| Scope | 7 | ||
| Principle | 7 | ||
| Reagents | 8 | ||
| Working stocks | 8 | ||
| Equipment | 8 | ||
| 18S rDNA Taqman Procedure | 8 | ||
| Mastermix | 8 | ||
| PCR | 9 | ||
| gp60 SYTO9 Procedure | 10 | ||
| Mastermix | 10 | ||
| PCR | 11 | ||
| Referenced Documents | 11 | ||
| 2. PCR DETECTION OF AMMONIA OXIDISING PROKARYOTES | 12 | ||
| Preface and Foreword | 12 | ||
| Health and Safety Information | 12 | ||
| Mandatory Safety Requirements | 12 | ||
| Hazard Warnings | 12 | ||
| Hazard Risk Assessment | 13 | ||
| Performance Characteristics | 14 | ||
| Scope | 14 | ||
| Principle | 14 | ||
| Reagents | 15 | ||
| Working stocks | 15 | ||
| Equipment | 16 | ||
| DNA extraction | 16 | ||
| Pre-treatment of samples before DNA Extraction | 16 | ||
| DNA Extraction with QIAGEN genomic DNA kit | 16 | ||
| PCR Procedures | 18 | ||
| Mastermix | 18 | ||
| PCR | 19 | ||
| Referenced Documents | 20 | ||
| 3. PCR DETECTION OF MICROCYSTIS AND POTENTIAL MICROCYSTIN PRODUCING CYANOBACTERIA | 21 | ||
| Preface and Foreword | 21 | ||
| Health and Safety Information | 21 | ||
| Mandatory Safety Requirements | 21 | ||
| Hazard Warnings | 21 | ||
| Hazard Risk Assessment | 21 | ||
| Performance Characteristics | 22 | ||
| Scope | 23 | ||
| Principle | 23 | ||
| Reagents | 24 | ||
| Working stocks | 24 | ||
| Equipment | 24 | ||
| 16S rDNA Taqman Procedure | 25 | ||
| Mastermix | 25 | ||
| PCR | 25 | ||
| mcyE SYTO9 Procedure | 26 | ||
| Mastermix | 26 | ||
| PCR | 27 | ||
| Referenced Documents | 27 | ||
| 4. PCR DETECTION OF ADENOVIRUS | 28 | ||
| Preface and Foreword | 28 | ||
| Health and Safety Information | 28 | ||
| Mandatory Safety Requirements | 28 | ||
| Hazard Warnings | 28 | ||
| Hazard Risk Assessment | 29 | ||
| Performance Characteristics | 29 | ||
| Scope | 30 | ||
| Principle | 30 | ||
| Reagents | 31 | ||
| Working stocks | 31 | ||
| Equipment | 31 | ||
| Hexon Taqman Procedure | 31 | ||
| Mastermix | 31 | ||
| PCR | 32 | ||
| Referenced Documents | 32 |