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Book Details
Abstract
The Activated Sludge (AS) Process is old technology but is still widely adopted worldwide for its convenience and simplicity: an impressive number (many hundred of thousands) of this kind of system are in operation. Occasionally, problems such as bulking and foaming occur, causing regulation violations and large investment is often required immediately to control them. For this reason, an intense research effort has been made during the last few decades to face these problems, and this report details the work undertaken by the IWA Specialist Group on Activated Sludge Population Dynamics. This Scientific and Technical Report describes the main reasons fslyuor the growth of the different filamentous microorganisms in activated sludge, and the biological molecular tools available today for the identification of the main biomass components. The wide range of experiences around the world is documented and the methods to avoid the proliferation of these organisms are presented and critically reviewed. Activated Sludge Separation Problems consists of seven chapters, presenting up-to-date technical and scientific aspects of these processes. Scientific and Technical Report No. 16
Table of Contents
Section Title | Page | Action | Price |
---|---|---|---|
Contents | 6 | ||
1.0 Wastewater characterization | 16 | ||
1.1 INTRODUCTION | 16 | ||
1.2 GROSS PARAMETERS | 17 | ||
1.3 PHYSICAL PROPERTIES | 18 | ||
1.4 ORGANIC MATTER | 18 | ||
1.5 INORGANIC MATTER | 20 | ||
1.6 BIODEGRADABILITY | 20 | ||
1.6.1 COD, Nitrogen fractionation | 20 | ||
1.6.2 Evaluation methods | 22 | ||
1.6.2.1 Carbonaceous substrate (COD) | 22 | ||
1.6.2.2 Nitrogen and phosphorus | 24 | ||
1.7 REFERENCES | 24 | ||
2.0 The Microbiology of Activated Sludge Process | 26 | ||
2.1 INTRODUCTION | 26 | ||
2.2 MICROORGANISMS IN ACTIVATED SLUDGE | 27 | ||
Bacteria: cell structure | 27 | ||
Inclusions | 29 | ||
2.3 THE IDENTIFICATION OF BACTERIA | 30 | ||
Conventional taxonomy | 30 | ||
Molecular taxonomy | 30 | ||
Molecular characterization of mixed biomass by molecular probes | 32 | ||
2.4 FILAMENTOUS BACTERIA | 32 | ||
2.5 MICROTHRIX PARVICELLA | 37 | ||
Identification of M. parvicella | 37 | ||
Physiology of M. parvicella | 37 | ||
2.6 THIOTHRIX | 38 | ||
2.7 NOSTOCOIDA LIMICOLA | 38 | ||
2.8 POLYPHOSPHATE ACCUMULATING ORGANISMS (PAO) | 39 | ||
2.9 GLYCOGEN ACCUMULATING ORGANISMS (GAO) | 39 | ||
2.10 NITRIFIERS | 40 | ||
2.11 DENITRIFIERS | 40 | ||
2.12 QUANTITATIVE ESTIMATION OF FILAMENTOUS BACTERIA IN ACTIVATED SLUDGE | 40 | ||
2.13 FISH ANALYSIS | 41 | ||
Sample fixation | 42 | ||
Paraformaldehyde fixation (Gram negative cells): | 42 | ||
Ethanol fixation (Gram positive cells): | 42 | ||
Quick sample fixation | 42 | ||
Standard protocol for hybridization on slides | 43 | ||
Hybridization buffer composition | 45 | ||
Washing buffer composition | 45 | ||
Sample examination by epifluorescent microscopy | 45 | ||
2.14\tREFERENCES | 46 | ||
3.0 AS Separation problems | 50 | ||
3.1 INTRODUCTION | 50 | ||
3.2 “WELL-SETTLING” ACTIVATED SLUDGE | 51 | ||
3.2.1 Requirements | 51 | ||
3.2.1.1\tMeasurement of settling and thickening properties | 52 | ||
3.2.2 Microscopic features of well settling activated sludge | 54 | ||
3.3 ACTIVATED SLUDGE SEPARATION PROBLEMS | 54 | ||
3.3.1 Poor floc microstructure | 54 | ||
3.3.1.1 Dispersed growth | 54 | ||
3.3.1.2 Unsettleable microflocs | 55 | ||
3.3.1.3 Viscous bulking | 56 | ||
3.3.2 Poor floc macrostructure | 57 | ||
3.3.2.1 Filamentous bulking | 57 | ||
3.3.2.2 Foaming caused by filamentous microorganisms | 58 | ||
3.3.3 Other reasons | 60 | ||
3.3.3.1 Rising sludge | 60 | ||
3.4 SUMMARY | 61 | ||
3.5 REFERENCES | 61 | ||
4.0 Aeration tank and secondary clarifier as one system | 62 | ||
4.1 INTRODUCTION | 62 | ||
4.2 \tAERATION TANK AND SECONDARY CLARIFIER INTERACTIONS | 63 | ||
4.2.1 Activated sludge process | 63 | ||
4.2.2 Secondary clarifier | 64 | ||
4.2.2.1 General performance, thickening function | 64 | ||
4.2.3 Separation function | 65 | ||
4.2.4 BOD5 | 65 | ||
4.2.5 COD | 65 | ||
4.2.6 Nitrogen and phosphorus | 66 | ||
4.3\tTHE IMPACT OF AERATION BASIN EQUIPMENT AND OPERATION ON THE PERFORMANCE OF SECONDARY CLARIFIERS | 66 | ||
4.3.1 Mechanical vs. diffused-air aeration | 66 | ||
4.3.2 Mixed liquor mixing | 67 | ||
4.3.3 Degasification and reflocculation in aeration tanks | 67 | ||
4.4 FEATURES OF SECONDARY CLARIFIER CONSTRUCTION | 68 | ||
4.4.1 Inlet structure with a flocculation zone | 68 | ||
4.4.1.1 Principles of a flocculation zone | 68 | ||
4.4.1.2 Conventional flocculation zones with tangential flow regime | 69 | ||
4.4.1.3 Novel flocculation zone with deflectors and a vertical flow regime | 69 | ||
4.4.2 Outlet structure | 70 | ||
4.4.2.1 Peripheral vs. internal effluent launders | 70 | ||
4.4.2.1.1 Circular clarifier, peripheral effluent launder (with inboard weir) | 70 | ||
4.4.2.1.2 Circular clarifier, internal effluent launder (with inset double-sided weirs) | 71 | ||
4.4.2.1.3 Effluent launders in rectangular clarifiers with longitudinal flow | 71 | ||
4.4.3 Scum baffles | 71 | ||
4.5 EFFICIENT SCUM REMOVAL FROM THE SURFACE OF SECONDARY CLARIFIERS | 72 | ||
4.5.1 Conventional scum boxes | 72 | ||
4.5.2 “Travelling” scum boxes | 73 | ||
4.5.3 Pneumatic systems | 74 | ||
4.6 REMOVAL OF SETTLED AND THICKENED SLUDGE | 74 | ||
4.6.1 Effect on the final effluent quality | 74 | ||
4.6.2 Mechanical scrapers | 75 | ||
4.6.3 Vacuum sludge removal | 75 | ||
4.7 REFERENCES | 76 | ||
5.1 INTRODUCTION | 77 | ||
5.2 SPECIFIC CONTROL METHODS | 78 | ||
5.2.1 Bulking due to low F/M | 78 | ||
5.2.1.1 Continuous plug-flow reactors | 79 | ||
5.2.1.2 Selectors | 81 | ||
a) Design based on a biokinetic models | 81 | ||
b) Design based on empirical criteria | 82 | ||
c) Enhancing the selector effect: anoxic, anaerobic or compartmentalized selectors | 82 | ||
5.2.1.3 Sequencing batch reactors | 84 | ||
5.2.1.4 Role of storage phenomena in microbial competition in substrate gradient processes | 84 | ||
5.2.2 Bulking due to low nutrient concentration | 85 | ||
5.2.3 Bulking due to low dissolved oxygen concentrations | 86 | ||
5.2.4 Bulking due to fatty acids in the influent stream: control methods for Microthrix parvicella | 86 | ||
5.2.5 Microbial and enzymatic preparations | 87 | ||
5.3 NON SPECIFIC CONTROL METHODS | 87 | ||
5.3.1 Oxidising agents | 89 | ||
5.3.2 Weighting or flocculating agents | 91 | ||
5.3.3 New findings: use of alluminum salts to control Microthrix parvicella | 92 | ||
5.4 CONTROL OF NON-FILAMENTOUS BULKING (“VISCOUS BULKING”) | 92 | ||
5.5 AVOIDING POOR SETTLING PROPERTIES: ALTERNATIVE SEPARATIONS OF ACTIVATED SLUDGE | 92 | ||
6.0 Experience in various countries | 98 | ||
6.1 INTRODUCTION | 98 | ||
6.2 AUSTRALIA | 98 | ||
6.2.1 General situation | 98 | ||
6.2.2 Foaming filamentous bacteria in Australian Activated Sludge Plants | 100 | ||
6.2.3 Foaming and Bulking Filamentous Bacteria in Activated Sludge Plants | 102 | ||
6.2.4 Do the filamentous populations in activated sludge plants change with time? | 105 | ||
6.2.5 Which operational factor affect filamentous populations and consequently determine incidents of bulking and foaming | 105 | ||
6.2.6 Control Measures for Bulking and Foaming | 106 | ||
6.2.7 An Australian Case Study | 107 | ||
6.2.7.1 Plant Design Features | 107 | ||
6.2.7.2 Microscopic Features of the Biomass | 107 | ||
6.2.7.3 Bulking and Foaming Incidents | 108 | ||
6.2.8 Conclusions | 109 | ||
6.3 CZECH REPUBLIK | 109 | ||
6.3.1 Separation problems - situation up to 1980s | 109 | ||
6.3.2 Separation problems - situation up to mid-1990s | 110 | ||
6.3.3 WWTPs screening - 1995 - 2000 | 110 | ||
6.3.3.1 Methodology | 110 | ||
6.3.3.2 Microscopic analysis and sedimentation properties evaluation | 111 | ||
6.3.4 Results of separation problems and filamentous micro-organisms screening | 111 | ||
6.3.4.1 Filamentous micro-organism abundance | 111 | ||
6.3.4.2 Dominant filamentous microorganisms in sludge and foam | 112 | ||
6.3.5 Development of filamentous population in Czech activated sludge plants between 1997 - 1998 | 113 | ||
6.3.5.1 SVI and total abundance of filaments | 113 | ||
6.3.5.2 Dominant filamentous microorganisms in activated sludge mixed liquor and foams | 113 | ||
6.3.6 Screening of eight nutrient removal plants in 2000 | 116 | ||
6.3.6.1 Characterization of monitored WWTPs | 116 | ||
6.3.6.2 Activated sludges and settling properties | 116 | ||
6.3.6.3 Dominant filamentous microorganisms in activated sludge mixed liquor | 116 | ||
6.3.6.4 Biological foams | 117 | ||
6.3.7 FOAM CONTROL STRATEGIES | 117 | ||
6.3.7.1 Water sprays | 117 | ||
6.3.7.2 Skimming (mechanical removal) of foam | 118 | ||
6.3.7.3 Return activated sludge manipulation | 119 | ||
6.3.7.4 Manipulation of SRT | 119 | ||
6.3.7.5 Additional installation of baffles | 119 | ||
6.3.7.6 Initial contact zones (“selectors“) | 120 | ||
6.3.7.7 Chlorination of foam | 120 | ||
6.4 DENMARK, GREECE AND NETHERLANDS | 120 | ||
6.4.1 General situation | 120 | ||
6.4.2 Wastewater composition | 120 | ||
6.4.3 Process configurations | 121 | ||
6.4.4 Magnitude of bulking and foaming problems in Denmark, Greece and The Netherlands | 122 | ||
6.4.5 Settling properties in nutrient removal plants | 122 | ||
6.4.6 Effect of different process conditions on SVI and the size of the filamentous population | 125 | ||
6.4.7 Composition of the filamentous population | 127 | ||
6.4.8 Influence of wastewater composition, process configuration and operating parameters on filamentous microorganisms growth | 128 | ||
6.4.9 Effects of wastewater composition | 128 | ||
6.4.10 Effects of process configuration | 128 | ||
6.4.11 Experience with control measures applied | 129 | ||
6.4.12 Non specific control measures | 129 | ||
6.4.13 Specific control measures | 131 | ||
6.4.14 Conclusions | 132 | ||
6.5 FRANCE | 133 | ||
6.5.1 General situation | 133 | ||
6.5.2 Bulking and foaming characterization | 133 | ||
6.5.3 Bulking and foaming situation | 133 | ||
6.5.4 Dominant filamentous microorganisms in bulking and foaming sludges | 134 | ||
6.5.5 Filamentous microorganisms and relationship with plant operation | 134 | ||
6.5.6 Successful solutions for bulking and foaming control | 135 | ||
6.5.7 Contact zone as preventive solution | 136 | ||
6.5.7.1 Key points of contact zone design | 137 | ||
6.5.8 Control of bulking by Cl2 addition | 139 | ||
6.6 GERMANY/AUSTRIA | 141 | ||
6.6.1 General situation | 141 | ||
6.6.2 Bulking and scum in municipal and industrial wastewater treatment plants | 141 | ||
6.6.3 Biology and specific troubleshooting measures of filamentous bacteria | 143 | ||
6.6.4 Sulfur bacteria | 143 | ||
6.6.5 Gram-negative filamentous bacteria in highly load plants | 144 | ||
6.6.6 Experience with selectors for industrial wastes in Austria | 144 | ||
6.6.6.1 Dimensioning and construction of an aerobic selector | 144 | ||
6.6.6.2 Storage capacity and substrate removal after shock loads, maximum storage capacity | 146 | ||
6.6.6.3 Influence of SRT and F/M ratio on the substrate elimination in the selector | 146 | ||
6.6.6.4 Nutrient supply and substrate elimination | 146 | ||
6.6.6.5 Adaptation of mixed liquor to a selector | 147 | ||
6.6.6.6 Microbial growth and storage of substrate in the selector | 147 | ||
6.6.7 Gram-positive filamentous bacteria in low load WWTP | 148 | ||
6.6.7.1 M. parvicella | 148 | ||
6.6.7.2 Nocardioform actinomycetes | 149 | ||
6.6.8 Eikelboom morphotypes Nostocoida limicola, Types 0041/0675, 1851, and 0092 | 150 | ||
6.6.9 Non-specific control measures for sludge bulking and scum | 151 | ||
6.6.9.1 Addition of aluminum salts for phosphate precipitation | 151 | ||
6.6.9.2 Addition of layered silicates | 151 | ||
6.6.9.3 Addition of lime | 151 | ||
6.6.10 Decreasing the biomass of filamentous bacteria | 152 | ||
6.7 ITALY | 152 | ||
6.7.1 General situation | 152 | ||
6.7.2 Filament surveys | 152 | ||
6.7.2.1 Puglia Region, Southern Italy | 154 | ||
6.7.2.2 Lazio Region, Central Italy | 154 | ||
6.7.2.3 Industrial wastes, Northern Italy | 154 | ||
6.7.2.4 Survey on 167 Domestic plants. | 155 | ||
6.7.3 Control methods | 155 | ||
6.7.3.1 Non specific methods | 155 | ||
6.7.3.2 Specific methods | 155 | ||
6.7.4 Case studies at full scale plants | 155 | ||
6.7.4.1 Reduction of Type 021N by chlorination at the Bagnuolo (Reggio Emilia) municipal plant | 155 | ||
6.7.4.2 Controlling growth of Nocardia and M. parvicella by selectors at the Moncasale (Reggio Emilia) treatment plant | 156 | ||
6.7.4.3 Experiences at Varese – Olona plant (northern Italy) for controlling Nocardia and M. parvicella growth by reducing sludge age | 157 | ||
6.7.4.4 Type 021N and Thiothrix reduction by an anoxic selector at Area Nolana Treatment Plant | 157 | ||
6.7.4.5 Selector investigations on four large WWTP in Central Italy | 158 | ||
6.7.4.6 M. parvicella elimination by poly aluminium chloride at Fusina treatment plant | 158 | ||
6.8 JAPAN | 160 | ||
6.8.1 General situation | 160 | ||
6.8.2 Major Filaments Responsible for problems | 162 | ||
6.8.3 Research on Fundamental Aspects of Filamentous Bulking in Japan/Thailand | 162 | ||
6.8.4 Control Measures Applied | 163 | ||
6.8.5 Case Studies | 163 | ||
Acknowledgement | 164 | ||
6.9 SOUTH AFRICA | 164 | ||
6.9.1 General situations | 164 | ||
6.9.1.1 Extended aeration plants | 164 | ||
6.9.1.2 Conventional activated sludge | 165 | ||
6.9.1.3 Plants treating industrial wastes | 166 | ||
6.9.2 Plants specifically designed for biological nutrient removal (BNR) | 166 | ||
6.9.2.1 First generation plants | 166 | ||
6.9.3 Filamentous organisms in South African activated sludge plants | 166 | ||
6.9.4 Reasons for the growth of filamentous organisms in SA plants. | 167 | ||
6.9.5 Methods used for the control of bulking and foaming in South Africa | 168 | ||
6.9.5.1 Physical removal | 168 | ||
6.9.5.2 Use of toxicants | 169 | ||
6.9.5.3 Reduction of sludge age | 169 | ||
6.9.5.4 Elimination and prevention of surface trapping | 170 | ||
6.9.5.5 Increase in clarification capacity | 170 | ||
6.9.5.6 Adjustments of dissolved oxygen levels | 170 | ||
6.9.5.7 Improvements of reactor configuration | 170 | ||
6.9.6 A case study | 171 | ||
6.10 UNITED KINGDOM | 172 | ||
6.10.1 General situation | 172 | ||
6.10.2 Current situation | 173 | ||
6.10.3\tControl measures | 173 | ||
6.10.4\tCase history | 175 | ||
6.10.4.1 Background | 175 | ||
6.10.4.2 Solution Process | 177 | ||
6.10.5 Conclusion | 178 | ||
6.11 USA | 178 | ||
6.11.1\tGeneral situations | 178 | ||
6.11.2\tControl of filaments in activated sludge systems | 179 | ||
6.11.3\tOther solids separation problems | 182 | ||
6.11.4\tFoaming | 182 | ||
6.11.5\tViscous Bulking and Dispersed Growth | 184 | ||
6.11.6\tConclusions | 187 | ||
6.12\tREFERENCES | 187 |