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Book Details
Abstract
For courses in Microbiology Lab and Nursing and Allied Health Microbiology Lab
A Flexible Approach to the Modern Microbiology Lab
Easy to adapt for almost any microbiology lab course, this versatile, comprehensive, and clearly written manual is competitively priced and can be paired with any undergraduate microbiology text. Known for its thorough coverage, straightforward procedures, and minimal equipment requirements, the Eleventh Edition incorporates current safety protocols from governing bodies such as the EPA, ASM, and AOAC. The new edition also includes alternate organisms for experiments for easy customization in Biosafety Level 1 and 2 labs. New lab exercises have been added on Food Safety and revised experiments, and include options for alternate media, making the experiments affordable and accessible to all lab programs. Ample introductory material, engaging clinical applications, and laboratory safety instructions are provided for each experiment along with easy-to-follow procedures and flexible lab reports with review and critical thinking questions.
Table of Contents
| Section Title | Page | Action | Price |
|---|---|---|---|
| Cover | Cover | ||
| Contents | 7 | ||
| Preface | 10 | ||
| Laboratory Safety | 13 | ||
| Laboratory Protocol | 15 | ||
| Part 1: Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms | 17 | ||
| Introduction | 17 | ||
| Experiment 1: Culture Transfer Techniques | 23 | ||
| Principle | 23 | ||
| Materials | 25 | ||
| Procedure Lab One | 25 | ||
| Observations and Results Culture “A” | 27 | ||
| Observations and Results Culture “B” | 27 | ||
| Observations and Results S. marcescens | 28 | ||
| Experiment 2: Techniques for Isolation of Pure Cultures | 31 | ||
| Part A: Isolation of Discrete Colonies from a Mixed Culture | 31 | ||
| Principle | 31 | ||
| Materials | 33 | ||
| Procedure Lab One | 34 | ||
| Procedure Lab Two | 34 | ||
| Part B: Isolation of Pure Cultures from a Spread-Plate or Streak-Plate Preparation | 34 | ||
| Principle | 34 | ||
| Materials | 35 | ||
| Procedure Lab One | 35 | ||
| Procedure Lab Two | 35 | ||
| Observations and Results | 37 | ||
| Review Questions | 39 | ||
| Experiment 3: Cultural Characteristics of Microorganisms | 41 | ||
| Principle | 41 | ||
| Materials | 43 | ||
| Procedure Lab One | 43 | ||
| Procedure Lab Two | 43 | ||
| Observations and Results | 45 | ||
| Part 2: Microscopy | 47 | ||
| Introduction | 47 | ||
| Experiment 4: Microscopic Examination of Stained Cell Preparations | 49 | ||
| Principle | 49 | ||
| Materials | 54 | ||
| Procedure | 54 | ||
| Observations and Results | 55 | ||
| Review Questions | 56 | ||
| Experiment 5: Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount | 57 | ||
| Principle | 57 | ||
| Materials | 58 | ||
| Procedure | 58 | ||
| Observations and Results | 61 | ||
| Review Questions | 62 | ||
| Part 3: Bacterial Staining | 63 | ||
| Introduction | 63 | ||
| Experiment 6: Preparation of Bacterial Smears | 67 | ||
| Principle | 67 | ||
| Materials | 68 | ||
| Procedure | 68 | ||
| Observations and Results | 71 | ||
| Review Questions | 71 | ||
| Experiment 7: Simple Staining | 73 | ||
| Principle | 73 | ||
| Materials | 73 | ||
| Procedure | 74 | ||
| Observations and Results | 77 | ||
| Review Questions | 77 | ||
| Experiment 8: Negative Staining | 79 | ||
| Principle | 79 | ||
| Materials | 79 | ||
| Procedure | 79 | ||
| Observations and Results | 81 | ||
| Review Questions | 82 | ||
| Experiment 9: Gram Stain | 83 | ||
| Principle | 83 | ||
| Materials | 85 | ||
| Procedure | 85 | ||
| Observations and Results | 89 | ||
| Review Questions | 89 | ||
| Experiment 10: Acid-Fast Stain | 91 | ||
| Principle | 91 | ||
| Materials | 93 | ||
| Procedure | 93 | ||
| Observations and Results | 95 | ||
| Review Questions | 95 | ||
| Experiment 11: Differential Staining for Visualization of Bacterial Cell Structures | 97 | ||
| Part A: Spore Stain (Schaeffer-Fulton Method) | 97 | ||
| Principle | 97 | ||
| Materials | 99 | ||
| Procedure | 99 | ||
| Part B: Capsule Stain (Anthony Method) | 99 | ||
| Principle | 99 | ||
| Materials | 101 | ||
| Procedure | 101 | ||
| Observations and Results | 103 | ||
| Review Questions | 104 | ||
| Part 4: Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations | 107 | ||
| Introduction | 107 | ||
| Experiment 12: Nutritional Requirements: Media for the Routine Cultivation of Bacteria | 109 | ||
| Principle | 109 | ||
| Materials | 111 | ||
| Procedure Lab One | 111 | ||
| Procedure Lab Two | 111 | ||
| Observations and Results | 113 | ||
| Review Questions | 114 | ||
| Experiment 13: Use of Differential, Selective, and Enriched Media | 115 | ||
| Principle | 115 | ||
| Materials | 118 | ||
| Procedure Lab One | 118 | ||
| Procedure Lab Two | 119 | ||
| Observations and Results | 121 | ||
| Review Questions | 123 | ||
| Experiment 14: Physical Factors: Temperature | 125 | ||
| Principle | 125 | ||
| Materials | 127 | ||
| Procedure Lab One | 127 | ||
| Procedure Lab Two | 127 | ||
| Observations and Results | 129 | ||
| Review Questions | 129 | ||
| Experiment 15: Physical Factors: pH of the Extracellular Environment | 131 | ||
| Principle | 131 | ||
| Materials | 132 | ||
| Procedure Lab One | 132 | ||
| Procedure Lab Two | 132 | ||
| Observations and Results | 133 | ||
| Review Questions | 133 | ||
| Experiment 16: Physical Factors: Atmospheric Oxygen Requirements | 135 | ||
| Principle | 135 | ||
| Materials | 136 | ||
| Procedure Lab One | 136 | ||
| Procedure Lab Two | 136 | ||
| Observations and Results | 139 | ||
| Review Questions | 139 | ||
| Experiment 17: Techniques for the Cultivation of Anaerobic Microorganisms | 141 | ||
| Principle | 141 | ||
| Materials | 143 | ||
| Media | 143 | ||
| Equipment | 143 | ||
| Procedure Lab One | 143 | ||
| Procedure Lab Two | 144 | ||
| Observations and Results | 145 | ||
| Review Questions | 145 | ||
| Experiment 18: Serial Dilution–Agar Plate Procedure to Quantitate Viable Cells | 147 | ||
| Principle | 147 | ||
| Materials | 149 | ||
| Procedure Lab One | 151 | ||
| Procedure Lab Two | 152 | ||
| Observations and Results | 153 | ||
| Review Questions | 153 | ||
| Experiment 19: The Bacterial Growth Curve | 155 | ||
| Principle | 155 | ||
| Materials | 156 | ||
| Procedure Lab One | 157 | ||
| Procedure Lab Two | 157 | ||
| Observations and Results | 159 | ||
| Review Questions | 160 | ||
| Part 5: Biochemical Activities of Microorganisms | 163 | ||
| Introduction | 163 | ||
| Experiment 20: Extracellular Enzymatic Activities of Microorganisms | 165 | ||
| Principle | 165 | ||
| Materials | 167 | ||
| Procedure Lab One | 168 | ||
| Procedure Lab Two | 168 | ||
| Observations and Results | 169 | ||
| Review Questions | 170 | ||
| Experiment 21: Carbohydrate Fermentation | 171 | ||
| Principle | 171 | ||
| Materials | 173 | ||
| Procedure Lab One | 174 | ||
| Procedure Lab Two | 174 | ||
| Observations and Results | 175 | ||
| Review Questions | 175 | ||
| Experiment 22: Triple Sugar–Iron Agar Test | 177 | ||
| Principle | 177 | ||
| Materials | 178 | ||
| Procedure Lab One | 179 | ||
| Procedure Lab Two | 179 | ||
| Observations and Results | 181 | ||
| Review Questions | 181 | ||
| Experiment 23: IMViC Test | 183 | ||
| Part A: Indole Production Test | 184 | ||
| Principle | 184 | ||
| Materials | 184 | ||
| Procedure Lab One | 185 | ||
| Procedure Lab Two | 185 | ||
| Part B: Methyl Red Test | 185 | ||
| Principle | 185 | ||
| Materials | 185 | ||
| Procedure Lab One | 186 | ||
| Procedure Lab Two | 186 | ||
| Part C: Voges-Proskauer Test | 186 | ||
| Principle | 186 | ||
| Materials | 187 | ||
| Procedure Lab One | 187 | ||
| Procedure Lab Two | 187 | ||
| Part D: Citrate Utilization Test | 187 | ||
| Principle | 188 | ||
| Materials | 188 | ||
| Procedure Lab One | 188 | ||
| Procedure Lab Two | 189 | ||
| Observations and Results | 191 | ||
| Review Questions | 193 | ||
| Experiment 24: Hydrogen Sulfide Test | 195 | ||
| Principle | 195 | ||
| Materials | 196 | ||
| Procedure Lab One | 196 | ||
| Procedure Lab Two | 196 | ||
| Observations and Results | 197 | ||
| Review Questions | 197 | ||
| Experiment 25: Urease Test | 199 | ||
| Principle | 199 | ||
| Materials | 199 | ||
| Procedure Lab One | 199 | ||
| Procedure Lab Two | 200 | ||
| Observations and Results | 201 | ||
| Review Questions | 201 | ||
| Experiment 26: Litmus–Milk Reactions | 203 | ||
| Principle | 203 | ||
| Materials | 204 | ||
| Procedure Lab One | 206 | ||
| Procedure Lab Two | 206 | ||
| Observations and Results | 207 | ||
| Review Questions | 207 | ||
| Experiment 27: Nitrate Reduction Test | 209 | ||
| Principle | 209 | ||
| Materials | 210 | ||
| Procedure Lab One | 210 | ||
| Procedure Lab Two | 210 | ||
| Observations and Results | 211 | ||
| Review Questions | 211 | ||
| Experiment 28: Catalase Test | 213 | ||
| Principle | 213 | ||
| Materials | 213 | ||
| Procedure Lab One | 214 | ||
| Procedure Lab Two | 214 | ||
| Observations and Results | 215 | ||
| Review Questions | 215 | ||
| Experiment 29: Oxidase Test | 217 | ||
| Principle | 217 | ||
| Materials | 217 | ||
| Procedure Lab One | 218 | ||
| Procedure Lab Two | 218 | ||
| Observations and Results | 219 | ||
| Review Questions | 219 | ||
| Experiment 30: Utilization of Amino Acids | 221 | ||
| Part A: Decarboxylase Test | 221 | ||
| Principle | 221 | ||
| Materials | 222 | ||
| Procedure Lab One | 222 | ||
| Procedure Lab Two | 223 | ||
| Part B: Phenylalanine Deaminase Test | 223 | ||
| Principle | 223 | ||
| Materials | 224 | ||
| Procedure Lab One | 224 | ||
| Procedure Lab Two | 224 | ||
| Observations and Results | 225 | ||
| Review Questions | 225 | ||
| Experiment 31: Genus Identification of Unknown Bacterial Cultures | 227 | ||
| Principle | 227 | ||
| Materials | 229 | ||
| Procedure Lab One | 229 | ||
| Procedure Lab Two | 229 | ||
| Observations and Results | 231 | ||
| Part 6: The Protozoa | 233 | ||
| Introduction | 233 | ||
| Experiment 32: Free-Living Protozoa | 235 | ||
| Principle | 235 | ||
| Materials | 235 | ||
| Procedure | 235 | ||
| Observations and Results | 237 | ||
| Review Questions | 239 | ||
| Experiment 33: Parasitic Protozoa | 241 | ||
| Principle | 241 | ||
| Materials | 243 | ||
| Procedure | 245 | ||
| Observations and Results | 247 | ||
| Review Questions | 248 | ||
| Part 7: The Fungi | 249 | ||
| Introduction | 249 | ||
| Experiment 34: Cultivation and Morphology of Molds | 251 | ||
| Part A: Slide Culture Technique | 251 | ||
| Principle | 251 | ||
| Materials | 253 | ||
| Procedure Lab One | 253 | ||
| Procedure Lab Two | 253 | ||
| Part B: Mold Cultivation on Solid Surfaces | 253 | ||
| Principle | 254 | ||
| Materials | 254 | ||
| Procedure Lab One | 254 | ||
| Procedure Lab Two | 254 | ||
| Observations and Results | 255 | ||
| Review Questions | 257 | ||
| Experiment 35: Yeast Morphology, Cultural Characteristics, and Reproduction | 259 | ||
| Principle | 259 | ||
| Materials | 260 | ||
| Procedure Lab One | 261 | ||
| Procedure Lab Two | 261 | ||
| Procedure Lab Three | 261 | ||
| Observations and Results | 263 | ||
| Review Questions | 265 | ||
| Experiment 36: Identification of Unknown Fungi | 267 | ||
| Principle | 267 | ||
| Materials | 270 | ||
| Procedure Lab One | 270 | ||
| Procedure Lab Two | 270 | ||
| Observations and Results | 271 | ||
| Part 8: The Viruses | 273 | ||
| Introduction | 273 | ||
| Experiment 37: Cultivation and Enumeration of Bacteriophages | 277 | ||
| Principle | 277 | ||
| Materials | 278 | ||
| Procedure Lab One | 278 | ||
| Procedure Lab Two | 278 | ||
| Observations and Results | 281 | ||
| Review Questions | 281 | ||
| Experiment 38: Isolation of Coliphages from Raw Sewage | 283 | ||
| Principle | 283 | ||
| Materials | 283 | ||
| Procedure Lab One | 285 | ||
| Procedure Lab Two | 285 | ||
| Procedure Lab Three | 285 | ||
| Observations and Results | 287 | ||
| Review Questions | 287 | ||
| Experiment 39: Propagation of Isolated Bacteriophage Cultures | 289 | ||
| Principle | 289 | ||
| Materials | 289 | ||
| Procedure Lab One | 290 | ||
| Procedure Lab Two | 290 | ||
| Procedure Lab Three | 290 | ||
| Observations and Results | 291 | ||
| Review Questions | 291 | ||
| Part 9: Physical and Chemical Agents for the Control of Microbial Growth | 293 | ||
| Introduction | 293 | ||
| Experiment 40: Physical Agents of Control: Moist Heat | 295 | ||
| Principle | 295 | ||
| Materials | 297 | ||
| Procedure Lab One | 297 | ||
| Procedure Lab Two | 297 | ||
| Observations and Results | 299 | ||
| Review Questions | 299 | ||
| Experiment 41: Physical Agents of Control: Electromagnetic Radiations | 301 | ||
| Principle | 301 | ||
| Materials | 302 | ||
| Procedure Lab One | 302 | ||
| Procedure Lab Two | 302 | ||
| Observations and Results | 303 | ||
| Review Questions | 303 | ||
| Experiment 42: Chemical Agents of Control: Chemotherapeutic Agents | 305 | ||
| Synthetic Agents | 305 | ||
| Part A: The Kirby-Bauer Antibiotic Sensitivity Test Procedure | 306 | ||
| Principle | 306 | ||
| Materials | 308 | ||
| Procedure Lab One | 308 | ||
| Procedure Lab Two | 308 | ||
| Part B: Synergistic Effect of Drug Combinations | 308 | ||
| Principle | 309 | ||
| Materials | 310 | ||
| Procedure Lab One | 310 | ||
| Procedure Lab Two | 311 | ||
| Observations and Results | 313 | ||
| Review Question | 314 | ||
| Experiment 43: Determination of Penicillin Activity in the Presence and Absence of Penicillinase | 315 | ||
| Principle | 315 | ||
| Part A: MIC Determination Using a Spectrophotometer | 316 | ||
| Materials | 316 | ||
| Part 10: Microbiology of Food | 331 | ||
| Introduction | 331 | ||
| Experiment 45: Microbiological Analysis of Food Products: Bacterial Count | 333 | ||
| Principle | 333 | ||
| Materials | 333 | ||
| Procedure Lab One | 333 | ||
| Procedure Lab Two | 333 | ||
| Observations and Results | 335 | ||
| Review Questions | 336 | ||
| Experiment 46: Microbial Fermentation | 337 | ||
| Part A: Alcohol Fermentation | 337 | ||
| Principle | 337 | ||
| Materials | 338 | ||
| Procedure | 338 | ||
| Part B: Lactic Acid Fermentation | 339 | ||
| Principle | 339 | ||
| Materials | 339 | ||
| Procedure | 339 | ||
| Observations and Results for Alcohol Fermentation | 341 | ||
| Observations and Results for Lactic Acid Fermentation | 341 | ||
| Review Questions | 342 | ||
| Part 11: Microbiology of Water | 343 | ||
| Introduction | 343 | ||
| Experiment 47: Standard Qualitative Analysis of Water | 345 | ||
| Principle | 345 | ||
| Materials | 347 | ||
| Procedure Lab One | 347 | ||
| Procedure Lab Two | 348 | ||
| Procedure Lab Three | 349 | ||
| Procedure Lab Four | 349 | ||
| Observations and Results | 351 | ||
| Review Questions | 352 | ||
| Experiment 48: Quantitative Analysis of Water: Membrane Filter Method | 353 | ||
| Principle | 353 | ||
| Materials | 353 | ||
| Procedure Lab One | 354 | ||
| Procedure Lab Two | 356 | ||
| Observations and Results | 357 | ||
| Review Questions | 358 | ||
| Part 12: Microbiology of Soil | 359 | ||
| Introduction | 359 | ||
| Experiment 49: Microbial Populations in Soil: Enumeration | 361 | ||
| Principle | 361 | ||
| Materials | 361 | ||
| Procedure Lab One | 362 | ||
| Procedure Lab Two | 364 | ||
| Procedure Lab Three | 364 | ||
| Observations and Results | 365 | ||
| Review Questions | 365 | ||
| Experiment 50: Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates | 367 | ||
| Principle | 367 | ||
| Materials | 367 | ||
| Part A: Isolation of Antibiotic-Producing Microorganisms | 368 | ||
| Procedure Lab One | 368 | ||
| Part 13: Bacterial Genetics | 379 | ||
| Introduction | 379 | ||
| Experiment 52: Enzyme Induction | 381 | ||
| Principle | 381 | ||
| Materials | 382 | ||
| Procedure | 383 | ||
| Observations and Results | 385 | ||
| Review Questions | 385 | ||
| Experiment 53: Bacterial Conjugation | 387 | ||
| Principle | 387 | ||
| Materials | 388 | ||
| Procedure Lab One | 388 | ||
| Procedure Lab Two | 388 | ||
| Observations and Results | 391 | ||
| Review Questions | 391 | ||
| Experiment 54: Isolation of a Streptomycin-Resistant Mutant | 393 | ||
| Principle | 393 | ||
| Materials | 394 | ||
| Procedure Lab One | 394 | ||
| Procedure Lab Two | 394 | ||
| Procedure Lab Three | 394 | ||
| Observations and Results | 395 | ||
| Review Questions | 395 | ||
| Experiment 55: The Ames Test: A Bacterial Test System for Chemical Carcinogenicity | 397 | ||
| Principle | 397 | ||
| Materials | 398 | ||
| Procedure Lab One | 398 | ||
| Procedure Lab Two | 398 | ||
| Observations and Results | 401 | ||
| Review Questions | 401 | ||
| Part 14: Biotechnology | 403 | ||
| Introduction | 403 | ||
| Experiment 56: Bacterial Transformation | 405 | ||
| Principle | 405 | ||
| Materials | 407 | ||
| Procedure Lab One | 409 | ||
| Procedure Lab Two | 410 | ||
| Observations and Results | 411 | ||
| Review Questions | 412 | ||
| Experiment 57: Isolation of Bacterial Plasmids | 413 | ||
| Principle | 413 | ||
| Materials | 414 | ||
| Procedure | 414 | ||
| Observations and Results | 419 | ||
| Review Questions | 420 | ||
| Experiment 58: Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA | 423 | ||
| Principle | 423 | ||
| Materials | 424 | ||
| Procedure | 424 | ||
| Observations and Results | 429 | ||
| Review Questions | 432 | ||
| Part 15: Medical Microbiology | 433 | ||
| Introduction | 433 | ||
| Experiment 59: Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries | 435 | ||
| Principle | 435 | ||
| Materials | 435 | ||
| Procedure Lab One | 436 | ||
| Procedure Lab Two | 436 | ||
| Observations and Results | 437 | ||
| Review Questions | 437 | ||
| Experiment 60: Normal Microbial Flora of the Throat and Skin | 439 | ||
| Principle | 439 | ||
| Part A: Isolation of Microbial flora | 439 | ||
| Materials | 441 | ||
| Procedure Lab One | 441 | ||
| Procedure Lab Two | 442 | ||
| Part B: Effectiveness of Handwashing | 443 | ||
| Principle | 443 | ||
| Materials | 443 | ||
| Procedure Lab One | 444 | ||
| Procedure Lab Two | 445 | ||
| Observations and Results | 447 | ||
| Review Questions | 449 | ||
| Experiment 61: Identification of Human Staphylococcal Pathogens | 451 | ||
| Principle | 451 | ||
| Materials | 453 | ||
| Procedure Lab One | 454 | ||
| Procedure Lab Two | 455 | ||
| Observations and Results | 457 | ||
| Experiment 62: Identification of Human Streptococcal Pathogens | 459 | ||
| Principle | 459 | ||
| Materials | 462 | ||
| Procedure Lab One | 462 | ||
| Procedure Lab Two | 463 | ||
| Observations and Results | 465 | ||
| Review Questions | 465 | ||
| Experiment 63: Identification of Streptococcus pneumoniae | 467 | ||
| Principle | 467 | ||
| Materials | 468 | ||
| Procedure Lab One | 469 | ||
| Procedure Lab Two | 469 | ||
| Observations and Results | 471 | ||
| Review Questions | 471 | ||
| Experiment 64: Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems | 473 | ||
| Principle | 473 | ||
| Materials | 476 | ||
| Procedure Lab One | 477 | ||
| Procedure Lab Two | 478 | ||
| Observations and Results | 479 | ||
| Review Questions | 480 | ||
| Experiment 65: Isolation and Presumptive Identification of Campylobacter | 483 | ||
| Principle | 483 | ||
| Materials | 484 | ||
| Procedure Lab One | 484 | ||
| Procedure Lab Two | 484 | ||
| Observations and Results | 485 | ||
| Review Questions | 486 | ||
| Experiment 66: Microbiological Analysis of Urine Specimens | 487 | ||
| Principle | 487 | ||
| Materials | 490 | ||
| Procedure Lab One | 490 | ||
| Procedure Lab Two | 490 | ||
| Observations and Results | 491 | ||
| Review Questions | 492 | ||
| Experiment 67: Microbiological Analysis of Blood Specimens | 493 | ||
| Principle | 493 | ||
| Materials | 495 | ||
| Procedure Lab One | 495 | ||
| Procedure Lab Two | 496 | ||
| Observations and Results | 497 | ||
| Review Questions | 497 | ||
| Experiment 68: Species Identification of Unknown Bacterial Cultures | 499 | ||
| Principle | 499 | ||
| Materials | 499 | ||
| Procedure Lab One | 500 | ||
| Procedure Lab Two | 501 | ||
| Procedure Lab Three | 502 | ||
| Procedure Lab Four | 503 | ||
| Observations and Results | 505 | ||
| Part 16: Immunology | 507 | ||
| Introduction | 507 | ||
| Experiment 69: Precipitin Reaction: The Ring Test | 509 | ||
| Principle | 509 | ||
| Materials | 510 | ||
| Procedure | 510 | ||
| Observations and Results | 511 | ||
| Review Questions | 511 | ||
| Experiment 70: Agglutination Reaction: The Febrile Antibody Test | 513 | ||
| Principle | 513 | ||
| Materials | 514 | ||
| Procedure | 514 | ||
| Observations and Results | 517 | ||
| Review Questions | 518 | ||
| Experiment 71: Enzyme-Linked Immunosorbent Assay | 519 | ||
| Principle | 519 | ||
| Materials | 520 | ||
| Procedure | 520 | ||
| Observations and Results | 521 | ||
| Review Question | 521 | ||
| Experiment 72: Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures | 523 | ||
| Part A: Rapid Plasma Reagin Test for Syphilis | 523 | ||
| Principle | 523 | ||
| Materials | 524 | ||
| Procedure | 524 | ||
| Part B: Genital Herpes: Isolation and Identification of Herpes Simplex Virus | 525 | ||
| Principle | 525 | ||
| Materials | 525 | ||
| Procedure | 526 | ||
| Part C: Detection of Sexually Transmitted Chlamydial Diseases | 526 | ||
| Principle | 526 | ||
| Materials | 527 | ||
| Procedure | 527 | ||
| Observations and Results | 529 | ||
| Review Questions | 530 | ||
| Appendices | 531 | ||
| Appendix 1: Scientific Notation | 531 | ||
| Appendix 2: Methods for the Preparation of Dilutions | 533 | ||
| Appendix 3: Microbiological Media | 535 | ||
| Appendix 4: Biochemical Test Reagents | 541 | ||
| Appendix 5: Staining Reagents | 544 | ||
| Appendix 6: Experimental Microorganisms | 545 | ||
| Art & Photo Credits | 547 | ||
| Index | 549 |