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Practical Skills in Biomolecular Science 5th edn

Practical Skills in Biomolecular Science 5th edn

Rob Reed | Jonathan Weyers | Allan Jones

(2016)

Additional Information

Book Details

Abstract

If you are studying the biomolecular sciences - including biochemistry, biomedical sciences, biotechnology, genetics, microbiology and molecular biology - then this book will be an indispensable companion throughout the whole of your degree programme. It provides effective explanation and support for the development of a wide range of laboratory and data analysis skills that you will use time and again during the practical aspects of your studies. This book also gives you a solid grounding in the broader transferable skills, which are increasingly necessary to achieve a high level of academic success.


Table of Contents

Section Title Page Action Price
Cover Cover
Title Page iii
Copyright Page iv
Contents v
List of boxes viii
Preface x
List of abbreviations xi
Acknowledgements xiii
For the student xiv
Study and examination skills 1
1. The importance of transferable skills 3
2. Managing your time 9
3. Working with others 13
4. Taking notes from lectures and texts 17
5. Learning effectively 23
6. Revision strategies 30
7. Assignments and exams 35
8. Preparing your curriculum vitae 45
Information technology and learning resources 51
9. Finding and citing published information 53
10. Evaluating information 59
11. Using online resources 67
12. Bioinformatics – Internet resources 77
13. Using spreadsheets 83
14. Using word processors, databases and other packages 89
Communicating information 97
15. Organising a poster display 99
16. Giving a spoken presentation 104
17. General aspects of scientific writing 110
18. Writing essays 117
19. Reporting practical and project work 120
20. Writing literature surveys and reviews 125
Fundamental laboratory techniques 129
21. Essentials of practical work 131
22. Bioethics 134
23. Health and safety 142
24. Working with liquids 145
25. Basic laboratory procedures 151
26. Principles of solution chemistry 161
27. pH and buffer solutions 169
28. Introduction to microscopy 176
29. Setting up and using a light microscope 180
The investigative approach 189
30. Making measurements 191
31. SI units and their use 195
32. Scientific method and design of experiments 200
33. Making notes of practical work 208
34. Project work 215
Working with cells and tissues 221
35. Sterile technique 223
36. Culture systems and growth measurement 230
37. Collecting and isolating microbes 241
38. Identifying microbes 246
39. Naming microbes and other organisms 252
40. Working with animal and plant tissues and cells 257
41. Homogenisation and fractionation of cells and tissues 266
Analytical techniques 273
42. Calibration and its application to quantitative analysis 275
43. Immunological methods 281
44. Using stable isotopes 291
45. Using radioisotopes 297
46. Measuring light 306
47. Basic spectroscopy 310
48. Advanced spectroscopy and spectrometry 319
49. Centrifugation 326
50. Chromatography – separation methods 332
51. Chromatography – detection and analysis 343
52. Principles and practice of electrophoresis 349
53. Advanced electrophoretic techniques 360
54. Electroanalytical techniques 366
Assaying biomolecules and studying metabolism 377
55. Analysis of biomolecules: fundamental principles 379
56. Assaying amino acids, peptides and proteins 382
57. Assaying lipids 387
58. Assaying carbohydrates 393
59. Assaying nucleic acids and nucleotides 398
60. Protein purification 403
61. Enzyme studies 411
62. Membrane transport processes 422
63. Photosynthesis and respiration 429
Genetics 439
64. Mendelian genetics 441
65. Bacterial and phage genetics 448
66. Molecular genetics I – fundamental principles 457
67. Molecular genetics II – PCR and related applications 467
68. Molecular genetics III – genetic manipulation techniques 474
Analysis and presentation of data 481
69. Manipulating and transforming raw data 483
70. Using graphs 487
71. Presenting data in tables 499
72. Hints for solving numerical problems 504
73. Descriptive statistics 514
74. Choosing and using statistical tests 525
Index 539