BOOK
Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics - E-Book
Carl A. Burtis | David E. Bruns
(2014)
Additional Information
Book Details
Abstract
A condensed, easier-to-understand student version of the acclaimed Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 7th Edition uses a laboratory perspective in providing the clinical chemistry fundamentals you need to work in a real-world, clinical lab. Coverage ranges from laboratory principles to analytical techniques and instrumentation, analytes, pathophysiology, and more. New content keeps you current with the latest developments in molecular diagnostics. From highly respected clinical chemistry experts Carl Burtis and David Bruns, this textbook shows how to select and perform diagnostic lab tests, and accurately evaluate results.
- Authoritative, respected author team consists of two well-known experts in the clinical chemistry world.
- Coverage of analytical techniques and instrumentation includes optical techniques, electrochemistry, electrophoresis, chromatography, mass spectrometry, enzymology, immunochemical techniques, microchips, automation, and point of care testing.
- Learning objectives begin each chapter, providing measurable outcomes to achieve after completing the material.
- Key words are listed and defined at the beginning of each chapter, and bolded in the text.
- A glossary at the end of the book makes it quick and easy to look up definitions of key terms.
- More than 500 illustrations plus easy-to-read tables help you understand and remember key concepts.
- New chapters on molecular diagnostics include the principles of molecular biology, nucleic acid techniques and applications, and genomes and nucleic acid alterations, reflecting the changes in this rapidly evolving field.
- New content on clinical evaluation of methods, kidney function tests, and diabetes is added to this edition.
- NEW multiple-choice review questions at the end of each chapter allow you to measure your comprehension of the material.
- NEW case studies on the Evolve companion website use real-life scenarios to reinforce concepts.
Table of Contents
| Section Title | Page | Action | Price |
|---|---|---|---|
| Front Cover | Cover | ||
| Contents | IFC1 | ||
| Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics | iii | ||
| Copyright | iv | ||
| Dedication to Seventh Edition | v | ||
| Contributors | vii | ||
| Foreword | xiii | ||
| Preface | xv | ||
| Contents | xvii | ||
| Part I - Principles of Laboratory Medicine | 1 | ||
| Chapter 1 - Clinical Chemistry, Molecular Diagnostics, and Laboratory Medicine | 1 | ||
| Laboratory Medicine | 1 | ||
| Clinical Chemistry and Laboratory Medicine | 2 | ||
| Molecular Diagnostics | 3 | ||
| Ethical Issues in Laboratory Medicine | 3 | ||
| Review Questions | 5 | ||
| Part II Analytical Techniques and Instrumentation | 129 | ||
| Chapter 9 - Optical Techniques* | 129 | ||
| Photometry and Spectrophotometry | 131 | ||
| Instrumentation | 133 | ||
| Reflectance Photometry | 138 | ||
| Atomic Absorption Spectrophotometry | 138 | ||
| Fluorometry | 139 | ||
| Phosphorimetry | 146 | ||
| Luminometry | 146 | ||
| Nephelometry and Turbidimetry | 147 | ||
| Review Questions | 149 | ||
| References | 150 | ||
| Chapter 10 - Electrochemistry and Chemical Sensors* | 151 | ||
| Potentiometry | 152 | ||
| Voltammetry/Amperometry | 158 | ||
| Conductometry | 162 | ||
| Coulometry | 163 | ||
| Optical Chemical Sensors | 163 | ||
| Biosensors | 164 | ||
| Review Questions | 169 | ||
| References | 170 | ||
| Chapter 11 - Electrophoresis | 171 | ||
| Basic Concepts and Definitions | 172 | ||
| Theory of Electrophoresis | 172 | ||
| Description of Technique | 172 | ||
| Types of Electrophoresis | 175 | ||
| Technical Considerations | 180 | ||
| Review Questions | 181 | ||
| References | 182 | ||
| Chapter 12 - Chromatography* | 183 | ||
| Basic Concepts | 184 | ||
| Separation Mechanisms | 187 | ||
| Planar Chromatography | 189 | ||
| Column Chromatography | 190 | ||
| Qualitative and Quantitative Analyses | 198 | ||
| Review Questions | 200 | ||
| References | 201 | ||
| Chapter 13 - Mass Spectrometry* | 202 | ||
| Basic Concepts and Definitions | 203 | ||
| Instrumentation | 205 | ||
| Clinical Applications | 213 | ||
| Review Questions | 214 | ||
| References | 215 | ||
| Chapter 14 - Enzyme and Rate Analyses* | 216 | ||
| Basic Principles | 217 | ||
| Enzyme Kinetics | 223 | ||
| Analytical Enzymology | 229 | ||
| Review Questions | 234 | ||
| References | 235 | ||
| Chapter 15 - Immunochemical Techniques* | 236 | ||
| Basic Concepts and Definitions | 237 | ||
| Antigen-Antibody Binding | 238 | ||
| Qualitative Methods | 240 | ||
| Quantitative Methods | 242 | ||
| Other Immunochemical Techniques | 252 | ||
| Review Questions | 252 | ||
| References | 253 | ||
| Chapter 16 - Automation | 254 | ||
| Basic Concepts | 255 | ||
| Automation of Analytical Processes | 255 | ||
| Integrated Automation forthe Clinical Laboratory | 263 | ||
| Other Areas of Automation | 269 | ||
| Review Questions | 270 | ||
| References | 270 | ||
| Chapter 17 - Point-of-Care Instrumentation | 272 | ||
| Analytical and Technological Considerations | 273 | ||
| Implementation and Management Considerations | 280 | ||
| Review Questions | 284 | ||
| References | 285 | ||
| Part III Analytes | 286 | ||
| Chapter 18 - Amino Acids, Peptides, and Proteins* | 286 | ||
| Amino Acids | 287 | ||
| Peptides and Proteins | 292 | ||
| Analysis of Proteins | 293 | ||
| Plasma and Serum Proteins | 299 | ||
| Review Questions | 317 | ||
| References | 317 | ||
| Chapter 19 - Serum Enzymes | 318 | ||
| Basic Concepts | 319 | ||
| Muscle Enzymes | 319 | ||
| Liver Enzymes | 322 | ||
| Pancreatic Enzymes | 328 | ||
| Other Clinically Important Enzymes | 331 | ||
| Enzymes as Cardiovascular Risk Markers | 334 | ||
| Review Questions | 335 | ||
| References | 335 | ||
| Chapter 20 - Tumor Markers and Cancer Genes | 337 | ||
| Cancer | 339 | ||
| Clinical Applications | 339 | ||
| Evaluating Clinical Utility | 341 | ||
| Clinical Guidelines | 342 | ||
| Analytical Methods | 342 | ||
| Enzymes | 342 | ||
| Hormones | 347 | ||
| Oncofetal Antigens | 349 | ||
| Cytokeratins | 351 | ||
| Carbohydrate Markers | 352 | ||
| Blood Group Antigens | 354 | ||
| Proteins | 355 | ||
| Receptors | 357 | ||
| Circulating Tumor | 358 | ||
| Genetic and Molecular Markers | 358 | ||
| Other Molecular Tests | 362 | ||
| Microarray-Based Markers | 362 | ||
| Review Questions | 363 | ||
| Part IV Pathophysiology | 608 | ||
| Chapter 33 - Diabetes | 608 | ||
| Classification | 610 | ||
| Hormones that Regulate Blood Glucose Concentration | 611 | ||
| Measurement of Insulin, Proinsulin, C-Peptide,and Glucagon | 614 | ||
| Pathogenesis of Type 1 Diabetes Mellitus | 616 | ||
| Pathogenesis of Type 2 Diabetes Mellitus | 617 | ||
| Diagnosis of Diabetes | 618 | ||
| Chronic Complications of Diabetes Mellitus | 620 | ||
| Role of the Clinical Laboratory in Diabetes Mellitus | 620 | ||
| Self-Monitoring of Blood Glucose | 621 | ||
| Alternatives to Meters for Monitoring of Blood Glucose | 623 | ||
| Ketone Bodies | 623 | ||
| Glycated Proteins | 625 | ||
| Urinary Albumin Excretion | 629 | ||
| Review Questions | 630 | ||
| References | 631 | ||
| Chapter 34 - Cardiovascular Disease | 632 | ||
| Anatomy and Physiology of the Heart | 633 | ||
| Cardiac Disease | 635 | ||
| Cardiac Biomarkers | 638 | ||
| Review Questions | 649 | ||
| References | 650 | ||
| Chapter 35 - Kidney Disease* | 651 | ||
| Anatomy | 653 | ||
| Kidney Function | 655 | ||
| Kidney Physiology | 658 | ||
| Pathophysiology of Kidney Disease | 663 | ||
| Other Diseases of the Kidney | 671 | ||
| Renal Replacement Therapy | 675 | ||
| Review Questions | 678 | ||
| References | 679 | ||
| Chapter 36 - Physiology and Disorders of Water, Electrolyte, and Acid-Base Metabolism | 680 | ||
| Total Body Water—Volume and Distribution | 681 | ||
| Electrolytes | 682 | ||
| Acid-Base Physiology | 688 | ||
| Conditions Associated with Abnormal Acid-Base Status and Abnormal Electrolyte Composition of the Blood5,10,11 | 692 | ||
| References | 699 | ||
| Chapter 37 - Liver Disease* | 700 | ||
| Anatomy of the Liver | 702 | ||
| Biochemical Functions of the | 704 | ||
| Clinical Manifestations of Liver Disease | 707 | ||
| Diseases of the Liver | 710 | ||
| Diagnostic Strategy | 720 | ||
| Review Questions | 722 | ||
| References | 722 | ||
| Chapter 38 - Gastrointestinal and Pancreatic Diseases | 724 | ||
| Anatomy | 725 | ||
| The Digestive Process | 726 | ||
| Gastrointestinal Regulatory Peptides13 | 728 | ||
| Stomach, Intestinal, and Pancreatic Diseases and Disorders | 730 | ||
| Review Questions | 740 | ||
| References | 740 | ||
| Chapter 39 - Disorders of Bone and Mineral Metabolism* | 741 | ||
| Overview of Bone and Mineral Metabolism | 743 | ||
| Calcium | 744 | ||
| Phosphate | 749 | ||
| Magnesium | 751 | ||
| Hormones Regulating Mineral Metabolism | 753 | ||
| Integrated Control of Mineral Metabolism | 760 | ||
| Biochemical Markers of Bone Turnover | 761 | ||
| Metabolic Bone Diseases | 765 | ||
| Review Questions | 767 | ||
| References | 767 | ||
| Chapter 40 - Disorders of the Pituitary* | 769 | ||
| Hypothalamic Regulation2-5,8 | 772 | ||
| Hormones of the Adenohypophysis2-5,8 | 772 | ||
| Hormones of the Neurohypophysis2,7-10 | 779 | ||
| Assessment of Anterior Pituitary Lobe Reserve | 782 | ||
| Review Questions | 783 | ||
| References | 783 | ||
| Chapter 41 - Disorders of the Adrenal Cortex* | 785 | ||
| General Steroid Chemistry | 786 | ||
| Adrenocortical Steroids | 788 | ||
| Dynamic Tests of Adrenal Function | 793 | ||
| Laboratory Evaluation of Adrenocortical Function | 800 | ||
| Review Questions | 805 | ||
| References | 805 | ||
| Chapter 42 - Thyroid Disorders* | 806 | ||
| Thyroid Hormones | 808 | ||
| Thyroid Disorders | 812 | ||
| Review Questions | 822 | ||
| References | 822 | ||
| Chapter 43 - Reproduction-Related Disorders* | 824 | ||
| Male Reproductive Biology | 826 | ||
| Female Reproductive Biology | 830 | ||
| Infertility | 841 | ||
| Analytical Methodology | 843 | ||
| Review Questions | 846 | ||
| References | 847 | ||
| Chapter 44 - Pregnancy and Prenatal Testing* | 848 | ||
| Human Pregnancy9 | 850 | ||
| Maternal Adaptation | 853 | ||
| Functional Development of the Fetus | 854 | ||
| Maternal and Fetal Health Assessment | 855 | ||
| Complications of Pregnancy | 856 | ||
| Prenatal Screening for Fetal Defects | 859 | ||
| Analytical Methodology | 863 | ||
| Review Questions | 868 | ||
| References | 869 | ||
| Chapter 45 - Newborn Screening and Inborn Errors of Metabolism* | 870 | ||
| Inheritance Pattern of Metabolic Disorders | 872 | ||
| Newborn Screening | 872 | ||
| Inborn Errors of Metabolism | 873 | ||
| Diagnostic Tests for Inherited Disorders of Metabolism | 882 | ||
| Review Questions | 883 | ||
| References | 884 | ||
| Chapter 46 - Pharmacogenetics | 885 | ||
| Defining Pharmacogenetic Targets | 887 | ||
| Approaches to Pharmacogenetic Testing | 887 | ||
| Clinical Applications of Pharmacogenetic Testing | 888 | ||
| Drug Metabolism and Pharmacogenomics | 889 | ||
| Review Questions | 897 | ||
| References | 897 | ||
| Part V Molecular Diagnostics | 899 | ||
| Chapter 47 - Principles of Molecular Biology | 899 | ||
| Landmark Developments in Genetics and Molecular Diagnostics | 901 | ||
| The Essentials | 902 | ||
| Nucleic Acid Structure and Organization | 902 | ||
| Nucleic Acid Physiology and Functional Regulation | 906 | ||
| Genetics and Epigenetics | 909 | ||
| Beyond the Nuclear Genome | 911 | ||
| Understanding Our Genome | 912 | ||
| Review Questions | 913 | ||
| References | 914 | ||
| Chapter 48 - Nucleic Acid Techniques and Applications | 915 | ||
| Nucleic Acid Enzymes | 918 | ||
| Amplification Techniques | 919 | ||
| Detection Techniques | 924 | ||
| Discrimination Techniques | 924 | ||
| Review Questions | 944 | ||
| References | 945 | ||
| Chapter 49 - Genomes and Nucleic Acid Variations | 946 | ||
| Genomes and Nucleic Acid Variations | 947 | ||
| Review Questions | 950 | ||
| References | 950 | ||
| Part VI Reference Information | 951 | ||
| Chapter 50 - Reference Information for the Clinical Laboratory* | 951 | ||
| Glossary | 995 | ||
| Index | 1031 | ||
| IBC | IBC |