Additional Information
Book Details
Abstract
Updated and easy-to-use, Linne & Ringsrud's Clinical Laboratory Science: The Basics and Routine Techniques, 6th Edition delivers a fundamental overview of the laboratory skills and techniques essential for success in your classes and your career. Author Mary Louise Turgeon's simple, straightforward writing clarifies complex concepts, and a discipline-by-discipline approach helps you build the knowledge to confidently perform clinical laboratory tests and ensure accurate, effective results.
- Expert insight from respected educator and author Mary Louise Turgeon reflects the full spectrum of clinical laboratory science.
- Engaging full-color design and illustrations familiarize you with what you'll see under the microscope.
- Streamlined approach makes must-know concepts and practices more accessible.
- Broad scope provides an ideal introduction to clinical laboratory science at various levels, including MLS/MLT and Medical Assisting.
- Hands-on procedures guide you through the exact steps you'll perform in the lab.
- Learning objectives help you identify key chapter content and study more effectively.
- Case studies challenge you to apply concepts to realistic scenarios.
- Review questions at the end of each chapter help you assess your understanding and identify areas requiring additional study.
- A companion Evolve website provides convenient online access to procedures, glossary, audio glossary and links to additional information.
- Updated instrumentation coverage familiarizes you with the latest technological advancements in clinical laboratory science.
- Perforated pages make it easy for you to take procedure instructions with you into the lab.
- Enhanced organization helps you study more efficiently and quickly locate the information you need.
- Convenient glossary provides fast, easy access to definitions of key terms.
Table of Contents
| Section Title | Page | Action | Price |
|---|---|---|---|
| Front Cover\r | Cover | ||
| Linné & Ringsrud’s Clinical Laboratory Science: The Basics and Routine Techniques | iii | ||
| Copyright | iv | ||
| Contributor | v | ||
| About the Author | vi | ||
| Reviewers | vii | ||
| Dedication | ix | ||
| Preface | xi | ||
| Acknowledgments | xiii | ||
| Contents | xv | ||
| Part I - Basic Laboratory Techniques \r | 1 | ||
| Chapter 1 - FUNDAMENTALS OF THE CLINICAL LABORATORY | 3 | ||
| CLINICAL LABORATORY SCIENCE | 4 | ||
| HISTORY OF CLINICAL LABORATORY SCIENCE AS A PROFESSION | 4 | ||
| MEDICAL ETHICS | 5 | ||
| HEALTH CARE ORGANIZATIONS | 5 | ||
| LABORATORY MEDICINE (CLINICAL PATHOLOGY) | 8 | ||
| LABORATORY DEPARTMENTS OR DIVISIONS | 9 | ||
| CLINICAL LABORATORY STAFFING AND FUNCTIONS | 11 | ||
| PRIMARY ACCREDITING ORGANIZATIONS | 12 | ||
| EXTERNAL GOVERNMENT LABORATORY ACCREDITATION AND REGULATION | 13 | ||
| CLINICAL LABORATORY IMPROVEMENT AMENDMENTS OF 1988 | 14 | ||
| ALTERNATE SITES OF TESTING | 15 | ||
| PATIENT SPECIMENS | 17 | ||
| QUALITY ASSESSMENT | 17 | ||
| ISO STANDARDS IN CLINICAL LABORATORIES | 20 | ||
| MEDICAL-LEGAL ISSUES | 21 | ||
| THE NEWEST DIRECTIONS FOR LABORATORY TESTING | 21 | ||
| REFERENCES | 22 | ||
| BIBLIOGRAPHY | 22 | ||
| Chapter 2 - SAFETY IN THE CLINICAL LABORATORY | 25 | ||
| SAFETY STANDARDS AND GOVERNING AGENCIES | 26 | ||
| AVOIDING TRANSMISSION OF INFECTIOUS DISEASES | 31 | ||
| SAFE WORK PRACTICES FOR INFECTION CONTROL | 33 | ||
| ADDITIONAL LABORATORY HAZARDS | 43 | ||
| FINAL DECONTAMINATION OF WASTE MATERIALS | 48 | ||
| BASIC FIRST-AID PROCEDURES | 48 | ||
| REFERENCES | 50 | ||
| BIBLIOGRAPHY | 51 | ||
| Chapter 3 - PHLEBOTOMY: COLLECTING AND PROCESSING BLOOD | 53 | ||
| QUALITY ASSESSMENT | 54 | ||
| INFECTION CONTROL | 55 | ||
| SPECIMEN COLLECTION | 56 | ||
| CAPILLARY OR PERIPHERAL BLOOD COLLECTION BY SKIN PUNCTURE | 67 | ||
| CAPILLARY BLOOD COLLECTION | 69 | ||
| SPECIMENS: GENERAL PREPARATION | 71 | ||
| CHAIN-OF-CUSTODY SPECIMEN INFORMATION | 74 | ||
| REFERENCES | 74 | ||
| BIBLIOGRAPHY | 75 | ||
| Chapter 4 - SYSTEMS OF MEASUREMENT, LABORATORY EQUIPMENT, AND REAGENTS | 78 | ||
| SYSTEMS OF MEASUREMENT | 79 | ||
| LABORATORY PLASTICWARE AND GLASSWARE | 82 | ||
| LABORATORY BALANCES | 93 | ||
| LABORATORY CENTRIFUGES | 96 | ||
| LABORATORY REAGENT WATER | 99 | ||
| REAGENTS USED IN LABORATORYASSAYS | 101 | ||
| REFERENCES | 109 | ||
| BIBLIOGRAPHY | 109 | ||
| Chapter 5 - THE MICROSCOPE | 111 | ||
| DESCRIPTION | 112 | ||
| PARTS OF THE MICROSCOPE | 112 | ||
| CARE AND CLEANING OF THE MICROSCOPE | 117 | ||
| USE OF THE MICROSCOPE | 118 | ||
| OTHER TYPES OF MICROSCOPES (ILLUMINATION SYSTEMS) | 121 | ||
| DIGITAL IMAGING | 126 | ||
| BIBLIOGRAPHY | 127 | ||
| RELATED WEBSITES | 127 | ||
| Chapter 6 - BASIC AND NEW TECHNIQUES IN THE CLINICAL LABORATORY | 129 | ||
| PHOTOMETRY | 130 | ||
| ABSORBANCE SPECTROPHOTOMETRY | 130 | ||
| REFLECTANCE SPECTROPHOTOMETRY | 137 | ||
| NEPHELOMETRY | 139 | ||
| FLOW (CELL) CYTOMETRY | 140 | ||
| IMMUNOFLUORESCENT LABELING TECHNIQUES | 141 | ||
| MOLECULAR TECHNIQUES | 146 | ||
| ELECTROCHEMICAL METHODS | 149 | ||
| CHROMATOGRAPHY | 154 | ||
| ANALYTICAL TECHNIQUES FOR POINT-OF-CARE TESTING | 155 | ||
| BIBLIOGRAPHY | 155 | ||
| Chapter 7 - LABORATORY MATHEMATICS AND SOLUTION PREPARATION | 159 | ||
| SIGNIFICANT FIGURES | 159 | ||
| EXPONENTS | 160 | ||
| DENSITY AND SPECIFIC GRAVITY | 161 | ||
| EXPRESSIONS OF SOLUTION CONCENTRATION | 161 | ||
| PROPORTIONS AND RATIOS | 164 | ||
| CONCENTRATIONS OF SOLUTIONS | 164 | ||
| DILUTIONS | 164 | ||
| BIBLIOGRAPHY | 168 | ||
| Chapter 8 - QUALITY ASSESSMENT\rAND QUALITY CONTROL IN\rTHE CLINICAL LABORATORY | 171 | ||
| CLINICAL LABORATORY IMPROVEMENT AMENDMENTS | 172 | ||
| VOLUNTARY ACCREDITING ORGANIZATIONS | 172 | ||
| ISO 15189 | 172 | ||
| LEAN AND SIX SIGMA | 173 | ||
| QUALITY ASSESSMENT—ERROR ANALYSIS | 174 | ||
| QUALITY ASSESSMENT—PHASES OF TESTING | 174 | ||
| QUALITY ASSESSMENT DESCRIPTORS | 179 | ||
| QUALITY CONTROL STATISTICS | 181 | ||
| MONITORING QUALITY CONTROL | 183 | ||
| TESTING OUTCOMES | 187 | ||
| REFERENCES | 187 | ||
| BIBLIOGRAPHY | 188 | ||
| Chapter 9 - POINT-OF-CARE TESTING | 190 | ||
| POINT-OF-CARE TESTING | 190 | ||
| FECAL OCCULT BLOOD | 192 | ||
| HANDHELD EQUIPMENT | 195 | ||
| REFERENCES | 197 | ||
| BIBLIOGRAPHY | 197 | ||
| Chapter 10 - LABORATORY INFORMATION SYSTEMS AND AUTOMATION | 199 | ||
| OVERVIEW OF LABORATORY INFORMATION MANAGEMENT SYSTEMS | 200 | ||
| COMPONENTS OF A COMPUTER SYSTEM | 200 | ||
| COMMUNICATION AND NETWORK DEVICES | 203 | ||
| COMPUTER APPLICATIONS | 205 | ||
| PRIVACY OF INDIVIDUALLY IDENTIFIABLE HEALTH INFORMATION | 207 | ||
| FUTURE CONSIDERATIONS | 207 | ||
| OVERVIEW OF AUTOMATION | 208 | ||
| AUTOMATED ANALYZERS | 210 | ||
| MOLECULAR TESTING | 213 | ||
| REFERENCES | 214 | ||
| BIBLIOGRAPHY | 215 | ||
| Part II - Clinical Laboratory Specializations\r | 217 | ||
| Chapter 11 - Introduction to Clinical Chemistry | 219 | ||
| GLUCOSE AND GLUCOSE METABOLISM | 220 | ||
| DIABETES | 221 | ||
| ELECTROLYTES | 228 | ||
| ACID-BASE BALANCE AND BLOOD GASES | 234 | ||
| RENAL FUNCTION | 235 | ||
| URIC ACID | 240 | ||
| AMMONIA | 240 | ||
| LIPIDS | 241 | ||
| CARDIAC DISEASE | 244 | ||
| LIVER AND PANCREATIC TESTING | 247 | ||
| HORMONE ASSAYS | 252 | ||
| TUMOR MARKERS | 253 | ||
| THERAPEUTIC DRUG MONITORING | 255 | ||
| DRUGS OF ABUSE | 257 | ||
| REFERENCES | 259 | ||
| BIBLIOGRAPHY | 259 | ||
| Chapter 12 - Principles and Practice of Clinical Hematology | 264 | ||
| INTRODUCTION TO HEMATOLOGY | 265 | ||
| BLOOD CELL DEVELOPMENT | 273 | ||
| CLINICAL HEMATOLOGY | 274 | ||
| HEMOGLOBIN | 277 | ||
| HEMATOCRIT (PACKED CELL VOLUME) | 280 | ||
| RED BLOOD CELL INDICES | 284 | ||
| BLOOD CELL COUNTS | 286 | ||
| EXAMINATION OF THE PERIPHERAL BLOOD FILM | 291 | ||
| ADDITIONAL HEMATOLOGY PROCEDURES | 321 | ||
| REFERENCES | 331 | ||
| BIBLIOGRAPHY | 331 | ||
| Chapter 13 - Introduction to Hemostasis | 335 | ||
| HEMOSTATIC MECHANISM | 336 | ||
| QUANTITATIVE PLATELET DISORDERS | 337 | ||
| COAGULATION | 338 | ||
| PATHWAYS FOR COAGULATION CASCADE | 343 | ||
| FIBRINOLYSIS | 344 | ||
| NORMAL PROTECTIVE MECHANISMS AGAINST THROMBOSIS | 345 | ||
| TESTS FOR HEMOSTASISAND COAGULATION | 347 | ||
| REFERENCES | 355 | ||
| BIBLIOGRAPHY | 355 | ||
| Chapter 14 - Renal Physiology and Urinalysis | 358 | ||
| OVERVIEW OF URINALYSIS | 359 | ||
| COMPOSITION OF URINE | 365 | ||
| COLLECTION AND PRESERVATION OF URINE SPECIMENS | 366 | ||
| PHYSICAL PROPERTIES OF URINE | 371 | ||
| MICROSCOPIC ANALYSIS OF URINE SEDIMENT | 396 | ||
| CONSTITUENTS OF URINE SEDIMENT | 401 | ||
| REFERENCES | 432 | ||
| BIBLIOGRAPHY | 432 | ||
| Chapter 15 - Examination of Body Fluids and Miscellaneous Specimens | 437 | ||
| OVERVIEW OF BODY FLUIDS | 438 | ||
| CEREBROSPINAL FLUID | 438 | ||
| SEROUS FLUIDS (PERICARDIAL, PLEURAL, AND PERITONEAL) | 442 | ||
| SYNOVIAL FLUID | 445 | ||
| SEMINAL FLUID | 451 | ||
| AMNIOTIC FLUID | 453 | ||
| SALIVA | 454 | ||
| REFERENCE | 455 | ||
| BIBLIOGRAPHY | 455 | ||
| Chapter 16 - Introduction to Microbiology | 458 | ||
| INTRODUCTION TO MICROORGANISMS | 459 | ||
| CLASSIFICATION OF MICROORGANISMS: TAXONOMY | 461 | ||
| PROTECTION OF LABORATORY PERSONNEL, DECONTAMINATION, DISINFECTION, AND STERILIZATION | 462 | ||
| SPECIMENS FOR MICROBIOLOGICAL EXAMINATION | 465 | ||
| BASIC EQUIPMENT AND TECHNIQUES USED IN MICROBIOLOGY | 469 | ||
| IDENTIFICATION OF BACTERIA | 472 | ||
| URINE CULTURES | 492 | ||
| THROAT CULTURES | 494 | ||
| GENITOURINARY CULTURES | 497 | ||
| BLOOD CULTURES | 500 | ||
| ANTIMICROBIAL SUSCEPTIBILITY TESTS | 502 | ||
| QUALITY CONTROL IN THE MICROBIOLOGY LABORATORY | 505 | ||
| TESTS FOR FUNGI (MYCOLOGY) | 506 | ||
| TESTS FOR PARASITES (PARASITOLOGY) | 510 | ||
| REFERENCES | 516 | ||
| BIBLIOGRAPHY | 516 | ||
| Chapter 17 - Immunology and Serology | 520 | ||
| OVERVIEW OF IMMUNOLOGY AND SEROLOGY | 521 | ||
| ANTIGENS AND ANTIBODIES | 524 | ||
| PRIMARY RESPONSE | 526 | ||
| SECONDARY RESPONSE | 526 | ||
| COMPLEMENT | 528 | ||
| PRINCIPLES OF IMMUNOLOGIC AND SEROLOGIC METHODS | 528 | ||
| SPECIMENS FOR SEROLOGY AND IMMUNOLOGY | 531 | ||
| COMMON IMMUNOLOGIC AND SEROLOGIC TESTS | 534 | ||
| BIBLIOGRAPHY | 541 | ||
| Chapter 18 - Immunohematology and Transfusion Medicine | 543 | ||
| OVERVIEW OF BLOOD BANKING | 544 | ||
| BENEFITS AND REASONS FOR TRANSFUSION | 545 | ||
| ANTIGENS AND ANTIBODIES IN IMMUNOHEMATOLOGY | 556 | ||
| ABO RED BLOOD CELL GROUP SYSTEM | 562 | ||
| OTHER BLOOD GROUP SYSTEMS | 568 | ||
| ANTIHUMAN GLOBULIN REACTION (COOMBS’ TEST) | 568 | ||
| COMPATIBILITY TESTING AND CROSSMATCHING | 570 | ||
| HEMOLYTIC DISEASE OF THE FETUSAND NEWBORN (HDFN) | 575 | ||
| REFERENCES | 579 | ||
| BIBLIOGRAPHY | 579 | ||
| Appendix A - NAACLS CLINICAL\rASSISTANT COMPETENCIES | 581 | ||
| CORE MODULE | 581 | ||
| CHEMISTRY MODULE | 582 | ||
| DONOR ROOM COLLECTION/SCREENING AND COMPONENTPROCESSING MODULE | 582 | ||
| HEMATOLOGY MODULE | 583 | ||
| IMMUNOLOGY MODULE | 583 | ||
| MICROBIOLOGY MODULE | 584 | ||
| URINALYSIS MODULE | 584 | ||
| Appendix B - ENGLISH-SPANISH\rMEDICAL PHRASES FOR THE\rPHLEBOTOMIST | 585 | ||
| Appendix C - PREFIXES, SUFFIXES,\rAND STEM WORDS | 586 | ||
| Appendix D - ABBREVIATIONS | 589 | ||
| Appendix E - QUICK REFERENCE\rVALUES | 591 | ||
| Appendix F - NAACLS ESSENTIAL\rFUNCTIONS | 593 | ||
| ASCLS MISSION/VISIONSTATEMENT | 593 | ||
| Answers to Review Questions | 594 | ||
| Glossary | 602 | ||
| Index | 629 |