Abstract

Some Biological Techniques in Electron Microscopy discusses the problems that hinder or prevent progress in biological electron microscopy. This book explores the great potential of electron microscope for solving several pressing medical and biological research problems.
Organized into five chapters, this book starts with an overview of the primary goal of biological electron microscopy, which is the visualization of atoms in biological molecules and structures. This text proceeds with a discussion of the freezing methods of electron microscopy in which some of the artifacts producing steps of environmental techniques are eliminated. Other chapters consider the objective of electron microscopy to resolve successively small objects, in the limiting instance, single atoms in amorphous structures. The final chapter deals with processing tissues for electron microscopy wherein temperature, agitation, pressure, and humidity can be controlled throughout the operation.
Biologists, biophysicists, physicists, electron microscopists, and medical researchers will find this book extremely useful.